Effects of streptomycin administration on increases in skeletal muscle fiber permeability and size following eccentric muscle contractions.
Effects of streptomycin administration on increases in skeletal muscle fiber permeability and size following eccentric muscle contractions.
复制标题
链霉素给药对偏心肌肉收缩后骨骼肌纤维通透性和尺寸增加的影响。
DOI：
10.1002/ar.23770
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Onishi H.
中科院分区：
文献类型：
--
作者：
Hayao K.;Tamaki H.;Nakagawa K.;Tamakoshi K.;Takahashi H.;Yotani K.;Ogita F.;Yamamoto N.;Onishi H.
本研究旨在探讨链霉素(STR)对大鼠离心性收缩(ECC)后肌膜通透性及受损肌纤维组织形态特征的影响。18只7周龄雄性Fischer 344大鼠随机分为3组：对照组(CONT)、体外循环组(ECC)和体外循环加 + 组(ECC STR)。两个ECC组的胫前肌(TA)均受到电刺激，表现为ECC。埃文斯蓝染料(EBD)是与膜通透性增加相关的肌肉纤维损伤的标志，在TA肌肉采样前24小时注射。通过组织形态分析确定EBD阳性纤维的数量、肌纤维横截面积(CSA)和圆度。ECC干预导致EBD阳性纤维比例增加，CSA增大，圆度降低。EC + 组EBD阳性纤维的比例比ECC组低79%。而EBD阳性纤维的CSA和圆度在ECC组和ECC组之间无明显差异。这些结果表明，给予STR可以减少ECC后增加膜通透性的肌纤维的数量，但不能改善现有EBD阳性纤维的纤维肿胀程度。Anat Rec，301：1096-1102,2018。©2018威利期刊公司。
The purpose of this study was to investigate the preventive effect of streptomycin (Str) administration on changes in membrane permeability and the histomorphological characteristics of damaged muscle fibers following eccentric contraction (ECC ). Eighteen 7‐week‐old male Fischer 344 rats were randomly assigned to three groups: control (Cont), ECC, and ECC with Str (ECC + Str). The tibialis anterior (TA) muscles in both ECC groups were stimulated electrically and exhibited ECC. Evans blue dye (EBD), a marker of muscle fiber damage associated with increased membrane permeability, was injected 24 hr before TA muscle sampling. The number of EBD‐positive fibers, muscle fiber cross‐sectional area (CSA), and roundness were determined via histomorphological analysis. The ECC intervention resulted in an increased fraction of EBD‐positive fibers, a larger CSA, and decreased roundness. The fraction of EBD‐positive fibers was 79% lower in the ECC + Str group than in the ECC group. However, there was no difference in the CSA and roundness of the EBD‐positive fibers between the two ECC groups. These results suggest that Str administration can reduce the number of myofibers that increase membrane permeability following ECC, but does not ameliorate the extent of fiber swelling in extant EBD‐positive fibers. Anat Rec, 301:1096–1102, 2018. © 2018 Wiley Periodicals, Inc.