MicroRNA expression profiles in serous ovarian carcinoma
MicroRNA expression profiles in serous ovarian carcinoma
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DOI：
10.1158/1078-0432.ccr-07-1731
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发表时间：
2008-05-01
影响因子：
11.5
通讯作者：
Kim, Sunghoon
中科院分区：
文献类型：
--
作者：
Nam, Eun Ji;Yoon, Heejei;Kim, Sunghoon
目的：虽然microRNA最近被认为是基因表达的核糖核酸调节因子，但对浆液性卵巢癌中microRNA的表达谱知之甚少。我们评估了microRNA的表达和microRNA表达与浆液性卵巢癌预后之间的关系。实验设计：2000年12月至2003年9月，20例浆液性卵巢癌患者和8例良性子宫疾病患者接受了治疗。结果：与正常卵巢组织相比，浆液性卵巢癌组织中存在多种microRNA的差异表达，包括miR-21、miR-125 a、miR-125 b、miR-100、miR-145、miR-16和miR-99 a。此外，一些microRNA的表达水平与浆液性卵巢癌患者的生存率相关。miR-200、miR-141、miR-18 a、miR-93和miR-429的高表达以及let-7 b和miR-199 a的低表达与不良预后显著相关（P < 0.05）。结论：我们的结果表明microRNA的失调参与了卵巢癌的发生，并与浆液性卵巢癌的预后相关。
Purpose: Although microRNAs have recently been recognized as riboregulators of gene expression, little is known about microRNA expression profiles in serous ovarian carcinoma. We assessed the expression of microRNA and the association between microRNA expression and the prognosis of serous ovarian carcinoma.Experimental Design: Twenty patients diagnosed with serous ovarian carcinoma and eight patients treated for benign uterine disease between December 2000 and September 2003 were enrolled in this study. The microRNA expression profiles were examined using DNA microarray and Northern blot analyses.Results: Several microRNAs were differentially expressed in serous ovarian carcinoma compared with normal ovarian tissues, including miR-21, miR-125a, miR-125b miR-100, miR-145, miR-16, and miR-99a, which were each differentially expressed in >16 patients. In addition, the expression levels of some microRNAs were correlated with the survival in patients with serous ovarian carcinoma. Higher expression of miR-200, miR-141, miR-18a, miR-93, and miR-429, and lower expression of let-7b, and miR-199a were significantly correlated with a poor prognosis (P < 0.05).Conclusion: Our results indicate that dysregulation of microRNAs is involved in ovarian carcinogenesis and associated with the prognosis of serous ovarian carcinoma.