Large-Scale Femtoliter Droplet Array for Single Cell Efflux Assay of Bacteria
Large-Scale Femtoliter Droplet Array for Single Cell Efflux Assay of Bacteria
复制标题
用于细菌单细胞流出检测的大规模飞升液滴阵列
DOI:
10.1007/978-1-4939-7454-2_18
复制
发表时间:
2018
影响因子:
--
通讯作者:
Nishino Kunihiko
中科院分区:
文献类型:
--
作者:
Iino Ryota;Sakakihara Shouichi;Matsumoto Yoshimi;Nishino Kunihiko
Large-scale femtoliter droplet array as a platform for single cell efflux assay of bacteria is described. Device microfabrication, femtoliter droplet array formation and concomitant enclosure of single bacterial cells, fluorescence-based detection of efflux activity at the single cell level, and collection of single cells from droplet and subsequent gene analysis are described in detail.