STIMULATION OF CD5 ENHANCES SIGNAL TRANSDUCTION BY THE T-CELL ANTIGEN RECEPTOR
STIMULATION OF CD5 ENHANCES SIGNAL TRANSDUCTION BY THE T-CELL ANTIGEN RECEPTOR
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DOI：
10.1172/jci114402
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发表时间：
1990-01-01
影响因子：
15.9
通讯作者：
LEDBETTER, JA
中科院分区：
文献类型：
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作者：
IMBODEN, JB;JUNE, CH;LEDBETTER, JA
在将CD3单抗加入到先前用植物血凝素刺激的外周T细胞中后，肌醇磷酸在2分钟内以较快的速度产生，此后以较慢的速度产生。刺激CD5可以使CD3介导的肌醇磷酸酯的产生以快速的速度维持20分钟，并增加最初CD3介导的肌醇三磷酸的增加和细胞内钙的释放。因此，单抗对CD5的干扰增强了CD3-抗原受体复合体与肌醇磷脂途径的偶联能力。CD5的这种作用独立于CD5单抗对肌醇磷酸水平的任何直接影响。
After the addition of a CD3 monoclonal antibody to peripheral T cells that have been previously stimulated with phytohemagglutinin, inositol phosphates are produced at a rapid rate for 2 min and at a much slower rate thereafter. Stimulation of CD5 allows CD3-mediated production of inositol phosphates to be sustained at a brisk rate for > 20 min and augments the initial CD3-mediated increase in inositol trisphosphate and release of intracellular Ca2+. Thus, perturbation of CD5 by monoclonal antibody enhances the ability of the CD3-antigen receptor complex to couple to the inositol phospholipid pathway. This effect of CD5 is independent of any direct effect of the CD5 monoclonal antibody on the levels of inositol phosphates.