A novel isolate of Bacillus thuringiensis serovar leesis that specifically exhibits larvicidal activity against the moth-fly, Telmatoscopus albipunctatus
A novel isolate of Bacillus thuringiensis serovar leesis that specifically exhibits larvicidal activity against the moth-fly, Telmatoscopus albipunctatus
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DOI：
10.1016/s0723-2020(98)80018-0
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发表时间：
1998-03-01
影响因子：
3.4
通讯作者：
Ohba, M
中科院分区：
文献类型：
--
作者：
Higuchi, K;Saitoh, H;Ohba, M
一株从土壤中分离得到的编号为88 - KO - 14 - 45的菌株，属于苏云金芽孢杆菌利斯变种（H33血清型），对蛾蚋（Telmatoscopus albipunctatus，双翅目：毛蠓科）幼虫具有杀虫活性，但对库蚊和伊蚊以及鳞翅目昆虫的幼虫没有活性。纯化的伴孢晶体对蛾蚋幼虫的半数致死浓度（LC50）值为5.78微克/毫升，而在蛋白质浓度高达10毫克/毫升时，对骚扰库蚊（Culex pipiens molestus，双翅目：蚊科）幼虫没有致死作用。电子显微镜观察显示，伴孢晶体是被厚的、电子致密的包膜包围的均匀圆形体。在溶解的伴孢晶体中未检测到对绵羊红细胞的溶血活性。十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）显示，伴孢晶体由72、68、56和30千道尔顿的蛋白质组成。从免疫学角度来看，这些蛋白质与苏云金芽孢杆菌以色列变种的伴孢晶体蛋白无关，而菌株73 - E - 10 - 2（苏云金芽孢杆菌达姆施塔特变种）的一种70千道尔顿的蛋白质对针对88 - KO - 14 - 45蛋白质的抗体具有血清学活性。
A soil isolate designated 88-KO-14-45, belonging to Bacillus thuringiensis serovar leesis (H33), exhibited larvicidal activity against the moth-fly; Telmatoscopus albipunctatus (Diptera: Psychodidae), but not for larvae of the culicine and aedine mosquitoes and Lepidoptera. Purified parasporal inclusions had an LC50 value of 5.78 mu g/ml for the larval moth-fly but gave no mortality against larvae elf Culex pipiens molestus (Diptera: Culicidae) at protein concentrations up to 10 mg/ml. Electron microscopic observations revealed that the parasporal inclusions are homogeneous round shaped bodies enclosed with thick, electron dense envelopes. Haemolytic activity against sheep erythrocytes was nor detected in the solubilized inclusions. SDS-PAGE showed that the inclusions are composed of 72, 68, 56, and 30 kDa proteins. Immunologically these proteins were unrelated to the inclusion proteins of B. thuringiensis serovar israelensis, while a 70 kDa protein of the strain 73-E-10-2 (B. thuringiensis serovar darmstadiensis) was seroactive to antibodies against proteins of 88-KO-14-45.