Effect of Stationary-Phase Sorption of Organic Modifier from a Water-Rich Mobile Phase on Solute Retention by an ODS Bonded Phase.
Effect of Stationary-Phase Sorption of Organic Modifier from a Water-Rich Mobile Phase on Solute Retention by an ODS Bonded Phase.
复制标题
富水流动相中有机改性剂的固定相吸附对 ODS 键合相保留溶质的影响。
DOI：
10.1021/ac981095e
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发表时间：
1999
影响因子：
7.4
通讯作者：
F. Cantwell
中科院分区：
文献类型：
--
作者：
N. Felitsyn;F. Cantwell
将1-丁醇（BuOH）和桉叶醇（Eu）在Partisil 10 ODS-3上的吸附等温线绘制为在水溶液中吸附的浓度与活性的关系。前者遵循朗缪尔方程，后者遵循“在水相中溶解度有限的缔合双层等温线”方程。两个系列的BuOH和Eu的同时吸附实验也进行了。一个系列涉及测量的影响BuOH，作为有机改性剂在水中的溶剂，Eu作为溶质的吸附。在第二个系列中，BuOH和Eu的作用被颠倒。这些研究表明，当吸附被视为两个本体液相之间的分配，固定相的行为是一个理想的溶液，它是由C（18）链，吸附的有机改性剂，和吸附的溶质。结合BuOH的Langmuir等温线行为，这种理想的溶液行为支持BuOH吸附在固定相/流动相界面，其烃尾在固定相中的观点。
Sorption isotherms for 1-butanol (BuOH) and eucalyptol (Eu) on Partisil 10 ODS-3 are plotted as concentration sorbed versus activity in aqueous solution. The former follows the Langmuir equation and the latter follows the equation for an "associative bilayer isotherm with limited solubility in the aqueous phase". Two series of simultaneous sorption experiments of BuOH and Eu were also performed. One series involved measuring the influence of BuOH, as an organic modifier solvent in water, on the sorption of Eu as solute. In the second series, the roles of BuOH and Eu were reversed. These studies show that when sorption is treated as partitioning between two bulk liquid phases, the stationary phase behaves as an ideal solution which is composed of C(18) chains, sorbed organic modifier, and sorbed solute. Combined with the Langmuir isotherm behavior of BuOH, this ideal solution behavior supports the view that BuOH is sorbed at the stationary-phase/mobile-phase interface, with its hydrocarbon tail in the stationary phase.