A novel fluorescent probe for NAD-consuming enzymes

A novel fluorescent probe for NAD-consuming enzymes
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DOI:
10.1039/c1cc15499k
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发表时间:
2011-01-01
影响因子:
4.9
通讯作者:
Wagner, Gerd K.
Wagner, Gerd K.
中科院分区:
化学2区
文献类型:
--
作者:
Pergolizzi, Giulia;Butt, Julea N.;Wagner, Gerd K.

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一种新的荧光NAD衍生物作为底物被三种不同的NAD消耗酶处理。这种新的探针已被用于通过荧光变化以连续的形式监测酶活性,并且在一种情况下,直接可视化替代反应途径。
A novel, fluorescent NAD derivative is processed as substrate by three different NAD-consuming enzymes. The new probe has been used to monitor enzymatic activity in a continuous format by changes in fluorescence and, in one case, to directly visualize alternative reaction pathways.