A murine model of Madurella mycetomatis eumycetoma
A murine model of Madurella mycetomatis eumycetoma
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DOI：
10.1016/s0928-8244(03)00096-8
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发表时间：
2003-06-10
影响因子：
--
通讯作者：
Bakker-Woudenberg, IAJM
中科院分区：
文献类型：
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作者：
Ahmed, AO;van Vianen, W;Bakker-Woudenberg, IAJM
由马杜拉菌肿引起的真菌肿是流行地区的一个主要真菌学健康问题。我们用不同量的分枝杆菌菌丝体感染 BALB/c 小鼠（雄性或雌性），其中含有无菌土壤作为天然佐剂或弗氏不完全佐剂。小鼠在年龄和免疫状态方面存在差异。探索了腹膜内、皮下和静脉内接种并监测存活率。接种后以不同的时间间隔处死小鼠，检查特征性黑色颗粒的存在，并培养器官中的分枝杆菌。对感染的器官进行组织病理学检查。免疫功能健全的雄性小鼠与免疫功能低下的雌性小鼠一样易感，但死亡率更高。总之，开发了一种可重复的腹膜内足菌分枝杆菌感染小鼠模型，该模型在免疫功能正常的成年或年轻雌性小鼠中具有特征性黑色颗粒。尽管该实验模型并未模拟人类皮下分枝杆菌感染的宏观特征，但病变的组织病理学特征和黑色颗粒的形成清楚地代表了人类感染。该模型将有助于进一步研究真菌感染的发病机制以及预防和治疗。 (C) 2003 年欧洲微生物学会联合会。由 Elsevier Science B.V. 出版。保留所有权利。
Eumycetoma due to Madurella mycetomatis is a major mycological health problem in endemic areas. We infected BALB/c mice (male or female) with various amounts of M. mycetomatis mycelium, containing sterilized soil as a natural adjuvant or Freund's incomplete adjuvant. Mice differed with respect to age and immune status. Intraperitoneal, subcutaneous and intravenous inoculation was explored and survival was monitored. Mice were killed at various intervals after inoculation, checked for the presence of the characteristic black grains, and organs were cultured for M. mycetomatis. Infected organs were subjected to histopathological examination. Immunocompetent male mice were as susceptible as immunocompromised female mice, but showed higher mortality rates. In conclusion, a reproducible mouse model of intraperitoneal M. mycetomatis infection with characteristic black grains in immunocompetent adult or young female mice was developed. Although this experimental model does not simulate macroscopic features of the subcutaneous M. mycetomatis infection in humans, the histopathological characteristics of the lesions and the development of black grains are clearly representative for the human infection. This model will enable further studies on the pathogenesis as well as prevention and treatment of the fungal infection. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.