Recognition of bovine respiratory syncytial virus proteins by bovine CD8+ T lymphocytes
Recognition of bovine respiratory syncytial virus proteins by bovine CD8+ T lymphocytes
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DOI：
10.1046/j.1365-2567.2003.01566.x
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发表时间：
2003-02-01
期刊：
影响因子：
6.4
通讯作者：
Taylor, G
中科院分区：
文献类型：
--
作者：
Gaddum, RM;Cook, RS;Taylor, G
CD 8（+）T淋巴细胞在清除牛呼吸道合胞病毒（BRSV）中发挥重要作用，BRSV是一种重要的幼牛呼吸道病原体，与婴儿呼吸道合胞病毒（HRSV）具有许多流行病学和病理学特征。使用表达单个BRSV蛋白的重组牛痘病毒（rVV）和重组鸡痘病毒（rFPV）证明F、N和M2蛋白是主要组织相容性复合体（MHC）定义的牛中牛CD 8（+）T细胞识别的主要抗原。使用细胞毒性T淋巴细胞（CTL）试验或通过BRSV蛋白再刺激后干扰素-γ（IFN-γ）的产生分析CD 8（+）T细胞对BRSV蛋白的识别。在接种表达G蛋白的rVV并随后用BRSV攻毒的牛中观察到CD 8（+）T细胞对G蛋白的强识别。尽管不同I类单倍型牛的MHC基因表达数量存在差异，但这似乎不影响CD 8（+）T细胞对BRSV蛋白的识别。了解BRSV特异性CD 8（+）T细胞的抗原特异性将有助于对牛中BRSV特异性CD 8（+）T细胞记忆进行定性和定量分析，并有助于确保潜在疫苗诱导定性适当的CD 8（+）T细胞应答。
CD8(+) T lymphocytes play a major role in the clearance of bovine respiratory syncytial virus (BRSV), an important respiratory pathogen of young calves that shares many of the epidemiological and pathological features of human respiratory syncytial virus (HRSV) in infants. Recombinant vaccinia virus (rVV) and recombinant fowlpox virus (rFPV), expressing individual BRSV proteins, were used to demonstrate that the F, N and M2 proteins were the major antigens recognized by bovine CD8(+) T cells in major histocompatibility complex (MHC)-defined cattle. BRSV protein recognition by CD8(+) T cells was analysed using cytotoxic T lymphocyte (CTL) assays or by the production of interferon-gamma (IFN-gamma) following restimulation with BRSV proteins. Strong recognition of the G protein by CD8(+) T cells was observed in cattle that had been vaccinated with rVV expressing this protein and subsequently challenged with BRSV. Although there is variation in the number of expressed MHC genes in cattle with different class I haplotypes, this did not appear to influence BRSV protein recognition by CD8(+) T cells. Knowledge of the antigenic specificity of BRSV-specific CD8(+) T cells will facilitate the qualitative and quantitative analysis of BRSV-specific CD8(+) T-cell memory in cattle and help to ensure that potential vaccines induce a qualitatively appropriate CD8(+) T-cell response.