Intraspecific variation in the venom of the vermivorous cone snail Conus vexillum
Intraspecific variation in the venom of the vermivorous cone snail Conus vexillum
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DOI：
10.1016/j.cbpc.2011.06.019
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发表时间：
2011-11-01
影响因子：
3.9
通讯作者：
Strong, Peter N.
中科院分区：
文献类型：
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作者：
Abdel-Rahman, Mohamed A.;Abdel-Nabi, Ismail M.;Strong, Peter N.
蛋白质组学和生物化学测定的组合被用来检查从两个地点（赫尔加达和沙姆沙伊赫）在红海，埃及的芋螺vexillum毒液的变化。使用MALDI/TOF-MS，发现两个地点的毒液样本之间存在显着的种内变异。为了评估芋螺毒液的细胞毒性作用的可变性，从每个位置向小鼠注射相同剂量。在注射蛇毒后3、6、9和12 h，测定氧化应激生物标志物[丙二醛（MDA）、蛋白质羰基含量（PCC）]、抗氧化剂[谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）]、总抗氧化能力（TAC）和一氧化氮（NO）。结果表明，该毒能显著提高PCC、MDA、NO、GSH和CAT的含量，抑制SOD活性，降低TAC。毒理学数据表明，从赫尔加达获得的毒液比从沙姆沙伊赫获得的毒液更有效。可以得出以下结论：（1）来自不同地区的同一种球茎的毒液高度多样化（2）来自不同地区的毒液反映出毒液效力的明显差异（3）C.维克塞卢姆毒液可归因于其诱导氧化应激的能力。(C)2011 Elsevier Inc. All rights reserved.
A combination of proteomic and biochemical assays was used to examine variations in the venom of Conus vexillum taken from two locations (Hurgada and Sharm El-Shaikh) in the Red Sea, Egypt. Using MALDI/TOF-MS, a remarkable degree of intra-species variation between venom samples from both locations was identified. To evaluate variability in the cytotoxic effects of Conus venom, mice were injected with the same dose from each location. The oxidative stress biomarkers [malondialdehyde (MDA), protein carbonyl content (PCC)], antioxidants [glutathione (GSH), superoxide dismutase (SOD), catalase (CAT)], total antioxidant capacity (TAC) and nitric oxide (NO), were measured 3, 6, 9 and 12 h post venom injection. The venoms induced a significant increase in the levels of PCC, MDA, NO, GSH and CAT. The venoms significantly inhibited the activity of SOD and reduced the TAC. Toxicological data showed that the venom obtained from Hurgada was more potent than that obtained from Sharm El-Shaikh. It can be concluded that: (1) the venom of the same Corms species from different regions is highly diversified (2) the venoms from different locations reflect clear differences in venom potency and (3) the cytotoxic effects of C. vexillum venom can be attributed to its ability to induce oxidative stress. (C) 2011 Elsevier Inc. All rights reserved.