Solid-liquid interface method (SLIM): a new crystallization method for proteins.
Solid-liquid interface method (SLIM): a new crystallization method for proteins.
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DOI：
10.1016/j.bbrc.2009.06.086
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发表时间：
2009-09
影响因子：
3.1
通讯作者：
E. Brostromer;J. Nan;Lan-fen Li;X. Su
中科院分区：
文献类型：
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作者：
E. Brostromer;J. Nan;Lan-fen Li;X. Su
尽管理论、方法和技术取得了令人瞩目的进步，但结晶仍然是大分子结构测定的严重瓶颈。在这里，我们提出了一种用于蛋白质结晶的新型固液界面方法（SLIM），该方法基于结晶试剂的预添加和干燥，然后将蛋白质溶液分配到干燥的介质中以启动从固液界面的结晶。该方法不仅快速且易于执行，还允许使用浓度较低的蛋白质溶液来建立结晶试验。
Despite impressive advances in theories, methods and technologies, crystallization still remains a serious bottleneck in structural determination of macromolecules. Here we present a novel solid–liquid interface method (SLIM) for protein crystallization, based on the pre-adding and drying of a crystallization reagent, and thereafter the dispensing of a protein solution to the dried media to initiate crystallization from the solid–liquid interface. Not only quick and easy to perform, the method also allows for a less concentrated protein solution for setting up crystallization trials.