Growth and toxicity of Protogonyaulax tamarensis in axenic culture.
Growth and toxicity of Protogonyaulax tamarensis in axenic culture.
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塔玛原角藻在无菌培养中的生长和毒性。
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
T. Yasumoto
中科院分区：
文献类型：
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作者：
H. T. Singh;Y. Oshima;T. Yasumoto
在无菌条件下培养分离自大船渡湾的一种有毒甲藻Protogonyaulax tamarensis，以阐明共存细菌对该生物产毒率和毒素谱的影响。每一个细胞的毒性根据培养时间的变化，表明毒性的变异性与甲藻本身的生理条件，而不是与细菌。在无菌培养条件下，毒素产量远高于单藻培养，这可能是由于在前者条件下生长较慢的结果。在生长的最早阶段观察到高达170 x 10-6 MU/细胞的毒性;这与相同物种的野生运动细胞和包囊的毒性相当。在无菌条件下培养的细胞的毒素谱与单细胞培养的细胞的毒素谱基本相同。
One of the toxic dinoflagellates Protogonyaulax tamarensis isolated in Ofunato Bay was grown in axenic state to clarify the effects of coexistent bacteria on the toxin productivety and toxin profile of the organism. The toxicity per one cell changed according to the culture period, indicating that the variability of toxicity is associated with the physiological condition of the dinoflagellate itself, but not with the bacteria. The toxin yields in axenic culture were much higher than in unialgal culture, probably as the result of the slower growth in the former condition. A toxicity as high as 170 x 10-6 MU/cell was observed at the earliest stage of growth; this was comparable to those of the wild motile cells and cysts of the same species. The toxin profile of the cells cul tured in bacteria-free condition was essentially the same as that of unialgally cultured ones.