In vitro controlled release of antigen in dendritic cells using pH-sensitive liposome-polymeric hybrid nanoparticles.
In vitro controlled release of antigen in dendritic cells using pH-sensitive liposome-polymeric hybrid nanoparticles.
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DOI：
10.1016/j.polymer.2015.10.048
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发表时间：
2015-12-02
期刊：
影响因子：
4.6
通讯作者：
Zhang C
中科院分区：
文献类型：
--
作者：
Hu Y;Zhao Z;Ehrich M;Fuhrman K;Zhang C
构建了由pH敏感脂质壳和聚（乳酸-羟基乙酸）（PLGA）核组成的混合纳米粒（NP）。该混合NP具有120.1 ± 8.8 nm的平均尺寸和带正电荷的表面（ζ电位为14.2 ± 1.4 mV）。在pH为5.5的缓冲液中，杂交NP的脂质壳迅速崩解，这类似于树突状细胞（DC）中内体的酸性环境。在生理pH下，在24 h内，小于20%的封闭在pH敏感性杂合NP中的抗原被释放到人血清中，但在pH调节至5.5后，超过40%的封闭抗原在8 h内被释放。还观察到DC对pH敏感的杂合NP的快速摄取。发现在被DC摄取后，与常规杂交NP相比，pH敏感性杂交NP显示出更快的降解和抗原释放。
A hybrid nanoparticle (NP) consisting of a pH sensitive lipid shell and a poly(lactic-co-glycolic) acid (PLGA) core was constructed. This hybrid NP has a mean size of 120.1 ± 8.8 nm and positively charged surface (zeta potential of 14.2 ± 1.4 mV). The lipid shell of the hybrid NP was quickly disintegrated in buffer with a pH of 5.5, which resembles the acidic environment of endosomes in dendritic cell (DC). Less than 20% of the antigen enclosed in pH-sensitive hybrid NP was released into human serum at physiological pH within 24 h, but more than 40% of the enclosed antigen was released within 8 h after pH was adjusted to 5.5. Fast uptake of the pH sensitive hybrid NP by DC was also observed. It was found that pH sensitive hybrid NP displayed faster degradation and antigen release compared to regular hybrid NPs after uptake by DC.