Suppression of mast cell-mediated allergic reaction by Amomum xanthiodes
Suppression of mast cell-mediated allergic reaction by Amomum xanthiodes
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DOI：
10.1016/j.fct.2007.05.011
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发表时间：
2007-11-01
影响因子：
4.3
通讯作者：
Shin, Tae-Yong
中科院分区：
文献类型：
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作者：
Kim, Sang-Hyun;Lee, Soyoung;Shin, Tae-Yong
肥大细胞介导的速发型过敏反应与哮喘、过敏性鼻炎、鼻窦炎等多种过敏性疾病有关。肥大细胞的刺激启动脱粒过程，导致组胺和一系列炎症细胞因子等介质的释放。在本报告中，我们研究了砂仁（姜科）水提取物（AXE）对肥大细胞介导的过敏模型的影响，并研究了其可能的作用机制。 AX 抑制化合物 48/80 诱导的小鼠全身反应和血清组胺释放。 AX 减少免疫球蛋白 E (IgE) 介导的被动皮肤过敏反应。 AX 减少了化合物 48/80 激活的大鼠腹膜肥大细胞的组胺释放和细胞内钙。此外，AX 降低了 p38 丝裂原激活蛋白激酶 (MAPK) 的激活，但不降低细胞外信号调节激酶和 c-jun N 末端激酶，以及佛波醇 12-肉豆蔻酸 13-乙酸酯和钙离子载体 A23187 刺激的人肥大细胞中下游肿瘤坏死因子 (TNF)-α 的产生。我们的研究结果证明 AX 可以抑制肥大细胞衍生的过敏反应，并且细胞内钙、TNF-α 和 p38 MAPK 参与这些作用。 (C) 2007 Elsevier Ltd. 保留所有权利。
The mast cell-mediated immediate-type allergic reaction is involved in many allergic diseases such as asthma, allergic rhinitis and sinusitis. Stimulation of mast cells starts the process of degranulation resulting in release of mediators such as histamine and an array of inflammatory cytokines. In this report, we investigated the effect of aqueous extract of Amomum xanthiodes (Zingiberaceae) (AXE) on the mast cell-mediated allergy model and studied its possible mechanisms of action. AXE inhibited compound 48/80-induced systemic reactions and serum histamine release in mice. AXE decreased immunoglobulin E (IgE)-mediated passive cutaneous anaphylaxis reaction. AXE reduced histamine release and intracellular calcium from rat peritoneal mast cells activated by compound 48/80. Furthermore, AXE decreased the activation of p38 mitogen-activated protein kinase (MAPK) but not extracellular signal-regulated kinase and c-jun N-terminal kinase, and downstream tumor necrosis factor (TNF)-alpha production in phorbol 12-myristate 13-acetate and calcium ionophore A23187-stimulated human mast cells. Our findings provide evidence that AXE inhibits mast cell-derived allergic reactions, and that intracellular calcium, TNF-alpha, and p38 MAPK are involved in these effects. (C) 2007 Elsevier Ltd. All rights reserved.