INDUCED CELL-SURFACE EXPRESSION OF FUNCTIONAL ALPHA-2-BETA-1-INTEGRIN DURING MEGAKARYOCYTIC DIFFERENTIATION OF K562 LEUKEMIC-CELLS
INDUCED CELL-SURFACE EXPRESSION OF FUNCTIONAL ALPHA-2-BETA-1-INTEGRIN DURING MEGAKARYOCYTIC DIFFERENTIATION OF K562 LEUKEMIC-CELLS
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DOI：
10.1016/0014-4827(92)90400-3
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发表时间：
1992-09-01
影响因子：
3.7
通讯作者：
SANTORO, SA
中科院分区：
文献类型：
--
作者：
BURGER, SR;ZUTTER, MM;SANTORO, SA
以多能造血细胞株K562作为诱导整合素表达伴随分化的模型进行了研究。phorbol 12,13-dibutyrate诱导巨核细胞途径分化，hemin诱导红细胞途径分化。巨核细胞分化的诱导与细胞形态的改变以及细胞-细胞和细胞-基质的粘附和扩散增加有关。红系分化与形态学或黏附的改变无关。细胞表面IIb-IIIa和α2β1整合素的表达在phorbol处理后明显升高，而在hemin处理后明显降低。phorbolt处理过的K562细胞，而不是对照细胞或hemintreated细胞，以Mg2+依赖的方式粘附在胶原底物上，这种粘附被针对α2β1整合素的单克隆抗体特异性抑制。Northern blot分析显示，K562细胞向巨核细胞分化的同时α2整合素mRNA的表达增加，而β1亚基mRNA的表达水平没有变化。K562细胞提供了一种依赖于分化的、受调控的整合素表达模型，其表达根据所选择的分化途径而上调或下调。
The pluripotential hematopoietic cell line K562 was studied as a model of inducible integrin expression accompanying differentiation. Differentiation along the megakaryocytic pathway was induced with phorbol 12,13-dibutyrate and differentiation along the erythroid pathway with hemin. Induction of megakaryocytic differentiation was associated with changes in cell morphology and with increased cell-cell and cell-substrate adhesion and spreading. Erythroid differentiation was not associated with changes in morphology or adhesion. Cell surface expression of the IIb-IIIa andα2β1integrins increased markedly with phorbol treatment but decreased with hemin treatment. Phorboltreated K562 cells, but not control cells or hemintreated cells, adhered to collagen substrates in a Mg2+-dependent manner which was specifically inhibited by a monoclonal antibody directed against theα2β1integrin. Northern blot analysis revealed that megakaryocytic differentiation of K562 cells was accompanied byde novoexpression of theα2integrin mRNA with no change in the level of mRNA for theβ1subunit. K562 cells provide a model of differentiation-dependent, regulated integrin expression in which expression is up- or down-regulated depending upon the differentiation pathway selected.