HLA-DRB1 alleles of susceptibility and protection in Iranians with autoimmune hepatitis
HLA-DRB1 alleles of susceptibility and protection in Iranians with autoimmune hepatitis
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DOI：
10.1016/j.humimm.2016.01.007
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发表时间：
2016-04-01
期刊：
影响因子：
2.7
通讯作者：
Tajik, Nader
中科院分区：
文献类型：
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作者：
Baharlou, Rasoul;Faghihi-Kashani, Amirhossein;Tajik, Nader
自身免疫性肝炎（AIH）是一种罕见的自身免疫性肝病，病因不明。本研究的目的是确定伊朗 AIH 患者 HLA-DRB1 等位基因的频率，并调查 HLA 等位基因与不同类型疾病之间的关联。 54 名 AIH 患者和 100 名年龄和性别匹配的健康对照者通过聚合酶链反应序列特异性引物 (PCR-SSP) 技术进行低分辨率 HLA-DRB 分型。结果显示，与对照组相比，AIH 患者的 HLA-DRB1*03 和 DRB1*13 等位基因频率更高。然而，DRB1*11 在 AIH 患者中出现频率较低。在 I 型 AIH 患者中，HLA-DRB1*03、HLA-DRB1*04、HLA-DRB1*08 和 HLA-DRB1*13 是最常见的等位基因。而在 II 型中，最常见的等位基因是 HLA-DRB1*07 和 HLA-DRB1*13。血清阴性患者的HLA-DRB1*03和HLA-DRB3频率较高。相反，1型中HLA-DRB1*11、HLA-DRB1*15和HLA-DRB5的频率低于健康个体。这些发现表明 HLA-DRB 单倍型在伊朗人群中 AIH 易感性和保护中的作用。 (C) 2016 年美国组织相容性和免疫遗传学学会。由爱思唯尔公司出版。保留所有权利。
Autoimmune hepatitis (AIH) is an uncommon autoimmune liver disease of unknown etiology. The aim of this study was to determine the frequency of HLA-DRB1 alleles in Iranian patients with AIH and investigate the association between HLA alleles and the different types of the disease. Fifty-four AIH patients and 100 age- and sex-matched healthy controls were subjected to low resolution HLA-DRB typing performed by polymerase chain reaction-sequence-specific primers (PCR-SSP) technique. The results revealed higher frequencies of HLA-DRB1*03, and DRB1*13 alleles in patients with AIH compared to controls. However, DRB1*11 was less frequent in AIH patients. In type I AIH patients HLA-DRB1*03, HLA-DRB1*04, HLA-DRB1*08, and HLA-DRB1*13 were the most frequent alleles. While in type II, the most frequent alleles were HLA-DRB1*07 and HLA-DRB1*13. The seronegative patients showed more frequency of HLA-DRB1*03 and HLA-DRB3. In contrary, the frequency of HLA-DRB1*11, HLA-DRB1*15 and HLA-DRB5 in type 1 was less than healthy individuals. These findings indicate the role of HLA-DRB haplotypes in AIH susceptibility and protection, in the Iranian population. (C) 2016 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.