High mobility group box chromosomal protein 1 in patients with renal diseases
High mobility group box chromosomal protein 1 in patients with renal diseases
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DOI：
10.1159/000118942
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发表时间：
2008-01-01
影响因子：
--
通讯作者：
Matsuo, Seiichi
中科院分区：
文献类型：
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作者：
Sato, Fumihiko;Maruyama, Shoichi;Matsuo, Seiichi
背景/目的：高迁移率族蛋白1（HMGB 1）是一种核DNA结合蛋白，最近被认为是一种新的促炎细胞因子。本研究的目的是探讨HMGB 1在肾脏疾病患者中的意义。研究方法：采用酶联免疫吸附试验测定血清中HMGB 1浓度，并采用Western印迹法检测肾活检患者和健康对照者血清中HMGB 1抗体。还进行了HMGB 1的免疫组织化学。结果：肾活检患者血清HMGB 1阳性率明显高于对照组。抗中性粒细胞胞浆抗体相关性肾炎（ANCA-GN）和过敏性紫癜性肾炎患者HMGB 1阳性率明显增高。抗HMGB 1抗体的存在与血清HMGB 1的存在无关。免疫组化结果显示，HMGB 1在ANCA-GN和伊加肾病（IgAN）患者的肾小球和肾小球内的单个核细胞表达。亚组分析显示，在IgAN患者中，有新月体形成的患者比没有新月体形成的患者表现出更高的HMGB 1阳性倾向。结论：HMGB 1在接受肾活检的肾脏疾病患者的血清中表达，特别是在那些患有血管炎的患者中，包括ANCA-GN、过敏性紫癜性肾炎和伴肾小球新月体的IgAN。版权所有（C）2008 S. Karger AG，巴塞尔。
Background/Aim: The high mobility group box chromosomal protein 1 (HMGB1), a nuclear DNA-binding protein, has recently been recognized as a new proinflammatory cytokine. The purpose of this study was to examine the significance of HMGB1 in patients with renal diseases. Methods: HMGB1 concentrations in sera were measured by enzyme-linked immunosorbent assay, and antibodies against HMGB1 were examined by Western blotting in patients who underwent renal biopsies and in healthy controls. Immunohistochemistry for HMGB1 was also performed. Results: Serum HMGB1 was more likely to be positive in patients who underwent renal biopsies as compared with the controls. Patients with anti-neutrophil cytoplasmic antibody-related glomerulonephritis (ANCA-GN) and those with Henoch-Schonlein purpura nephritis showed a significantly higher tendency to be HMGB1 positive. The presence of anti-HMGB1 antibody was not associated with the presence of serum HMGB1. Immunohistochemistry revealed that HMGB1 was expressed in mononuclear cells in the interstitium or in the glomeruli of some patients with ANCA-GN or IgA nephropathy (IgAN). Subanalysis demonstrated that among patients with IgAN, those who had crescent formation showed a higher tendency to be HMGB1 positive than those who did not. Conclusions: HMGB1 was expressed in the sera of patients with renal diseases who underwent renal biopsies, especially among those who had vasculitis including ANCA-GN, Henoch-Schonlein purpura nephritis, and IgAN with glomerular crescents. Copyright (C) 2008 S. Karger AG, Basel.