Tumor idiotype vaccines. VIII. Analysis of protective idiotype in sera and hybridomas derived from tumor-bearing mice with long-term survival.
Tumor idiotype vaccines. VIII. Analysis of protective idiotype in sera and hybridomas derived from tumor-bearing mice with long-term survival.
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肿瘤独特型疫苗。
DOI：
10.1007/bf01526793
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Kohler,H
中科院分区：
文献类型：
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作者：
Chen,JJ;Kohler,H
在这项研究中，独特型阳性的抗肿瘤抗体（抗Id）的贡献，在保护性肿瘤免疫进行了研究。我们先前已经表明，在产生的各种抗Id中，并且被分型为肿瘤相关抗体（TAA）gp52的内部图像Ab2，只有2F10抗体诱导保护性免疫。荷瘤小鼠血清中2F10独特表位的增加与长期生存相关，而在生存期短的小鼠中，循环2F10独特型减少。从长期存活的荷瘤小鼠血清中纯化2F10+IG，并测定2F10+抗TAA抗体的量。仅约3%的2F10+抗体是2F10+抗TAA+。从具有自发肿瘤消退的2F10高小鼠产生杂交瘤。52个肿瘤特异性杂交瘤中只有2个是2F10+。这些结果表明，2F10疫苗接种诱导的保护作用可能不是由2F10+抗体直接介导的，而是通过刺激2F10特异性细胞免疫应答间接介导的。
In this study, the contribution of idiotype-positive antitumor antibodies (anti-Id) in protective tumor immunity was investigated. We have previously shown that among various anti-Id generated and typed as the internal-image Ab2 of the tumor-associated antibody (TAA) gp52, only 2F10 antibody induces protective immunity. Increase of the 2F10 idiotope in sera of tumor-bearing mice correlated with long-term survival, while in mice with short survival the circulating 2F10 idiotype decreased. 2F10+Ig were purified from sera of tumor-bearing mice with longterm survival and the amount of 2F10+anti-TAA antibodies was determined. Only about 3% of 2F10+antibodies are 2F10+anti-TAA+. Hybridomas were generated from a 2F10 high mouse with spontaneous tumor regression. Only 2 out of 52 tumor-specific hybridomas were 2F10+. These results suggest that the protective effect induced by 2F10 vaccination may not be directly mediated by 2F10+antibodies but indirectly through the stimulation of a 2F10-specific cellular immune response.