Improvement of the analysis of dansylated derivatives of polyamines and their conjugates by high-performance liquid chromatography
Improvement of the analysis of dansylated derivatives of polyamines and their conjugates by high-performance liquid chromatography
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DOI：
10.1016/s0021-9673(01)00841-x
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发表时间：
2001-06-15
影响因子：
4.1
通讯作者：
Legaz, ME
中科院分区：
文献类型：
--
作者：
Fontaniella, B;Mateos, JL;Legaz, ME
本文介绍了一种改善从青苔中分离的共轭多胺在PH分数中水解的方法，并根据衍生化的PH值对这些多胺的丹基化过程进行了优化。丹西化的多胺后来被高效液相色谱（HPLC）用梯度洗脱分离。水解共轭物需要在室温下进行酸处理，而不是通常所描述的在100度下进行酸处理。丹酰化在高pH值下得到改善，而从共轭物中去除酚类物质（主要是戊酸）需要低pH值。(C) 2001 Elsevier Science B.V.版权所有
The paper described a method for improving the hydrolysis of conjugated polyamines in PH fraction, isolated from the lichen Evernia prunastri, as well as the optimization of dansylation procedure of these polyamines on the basis of the pH value to which derivatization is achieved. Dansylated polyamines have been later separated by high-performance liquid chramatography (HPLC) using a gradient elution. Hydrolysis of conjugates requires acid treatment at room temperature rather than at 100 degreesC, as usually described. Dansylation is improved at high pH values, whereas removal of phenolics (mainly evernic acid), from the conjugates requires low pH values. (C) 2001 Elsevier Science B.V. All rights reserved.