The importance of activation of antihemophilic globulin and proaccelerin by traces of thrombin in the generation of intrinsic prothrombinase activity.
The importance of activation of antihemophilic globulin and proaccelerin by traces of thrombin in the generation of intrinsic prothrombinase activity.
复制标题
痕量凝血酶激活抗血友病球蛋白和加速素原在产生内在凝血酶原酶活性中的重要性。
DOI：
10.1182/blood.v21.2.221.221
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发表时间：
1963
期刊：
影响因子：
20.3
通讯作者：
Sara Beth Ames
中科院分区：
文献类型：
--
作者：
S. Rapaport;Sandra Schiffman;M. Patch;Sara Beth Ames
体外内源性凝血有两个主要耗时步骤。第一步的重要性-通过激活产物（AP）活性的产生触发内源性凝血-已被赞赏了几年。本文一直关注的第二个重要的耗时步骤的描绘-微量凝血酶的产生，通过激活抗血友病球蛋白（AHG，因子VIII）和促凝血素原（因子V），将内在的凝血过程转入高速档。已经提供的数据表明，当血浆含有AP、活化的AHG（AHG ′）、活化的促凝血酶原（促凝血酶原）和游离的血小板因子3样活性时，产生强大的内在凝血酶原酶活性所需的所有剩余反应都在抗凝后7至12秒内发生。很可能AHG和促凝血酶原必须被微量凝血酶激活，才能有效参与内源性凝血酶原酶活性的产生。
There are two major time-consuming steps in intrinsic clotting in vitro. The importance of the first step—the triggering of intrinsic clotting through the generation of activation product (AP) activity—has been appreciated for several years. This paper has been concerned with the delineation of the second important time-consuming step—the generation of a trace of thrombin, which by activating both anti-hemophilic globulin (AHG, factor VIII ) and proaccelerin (factor V ), shifts the intrinsic clotting process into high gear. Data have been presented which indicate that when plasma contains AP, activated AHG (AHG'), activated proaccelerin (accelerin ) and free platelet factor 3-like activity, all of the remaining reactions required to generate powerful intrinsic prothrombinase activity take place within 7 to 12 seconds after recalcification. It may well be that AHG and proaccelerin must be activated by minute traces of thrombin before they can participate effectively in the generation of intrinsic prothrombinase activity.