Programmed changes in the adult rat offspring caused by maternal protein restriction during gestation and lactation are attenuated by maternal moderate-low physical training
Programmed changes in the adult rat offspring caused by maternal protein restriction during gestation and lactation are attenuated by maternal moderate-low physical training
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DOI：
10.1017/s0007114512001316
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发表时间：
2013-02-14
影响因子：
3.6
通讯作者：
Leandro, Carol Gois
中科院分区：
文献类型：
--
作者：
Fidalgo, Marco;Falcao-Tebas, Filippe;Leandro, Carol Gois
研究了母亲进行中、低强度体育锻炼对围产期低蛋白饮食子代成年后发育、血糖稳态和瘦素水平的影响。雄性Wistar大鼠（150日龄）按母体分为4组：未训练组（NTp，n 8）、训练组（T-p，n 8）、低蛋白饮食训练组（NT + LPp，n 8）和低蛋白饮食训练组（T + LPp，n 8）。在交配前，受过训练的母亲进行了为期4周的适度体育训练（跑步机，5天/周，60分钟/天，65%VO2max）。在怀孕期间，运动的强度和持续时间逐渐减少（50-20分钟/天，65- 30%VO2max）。低蛋白饮食组在妊娠期和哺乳期接受8%的酪蛋白饮食，他们的同龄人接受17%的酪蛋白饮食。每周记录幼仔的出生体重和身体生长，直至第150天。评估空腹血糖、胆固醇、血清瘦素浓度、葡萄糖和胰岛素耐量试验。T-p动物的躯体和生化参数没有变化，而NT + LPp组显示出更大的腹围、高血脂、高胆固醇血症、葡萄糖耐受不良和更低的血浆瘦素。在T + LPp动物中，除血浆瘦素浓度外，所有这些改变均被逆转。总之，围产期低蛋白饮食对生长和发育，葡萄糖稳态和血清瘦素浓度的后代的影响在训练过的母亲的幼崽中减弱。
The effects of maternal moderate-low physical training on postnatal development, glucose homeostasis and leptin concentration in adult offspring subjected to a low-protein diet during the perinatal period were investigated. Male Wistar rats (aged 150 d old) were divided into four groups according to maternal group: untrained (NTp, n 8); trained (T-p, n 8); untrained with a low-protein diet (NT + LPp, n 8); trained with a low-protein diet (T + LPp, n 8). The trained mothers were subjected to a protocol of moderate physical training over a period of 4 weeks (treadmill, 5 d/week, 60 min/d, at 65% VO2max) before mating. At pregnancy, the intensity and duration of exercise was progressively reduced (50-20 min/d, at 65-30% VO2max). The low-protein diet groups received an 8% casein diet, and their peers received a 17% casein diet during gestation and lactation. The pups' birth weight and somatic growth were recorded weekly up to the 150th day. Fasting blood glucose, cholesterol, serum leptin concentration, glucose and insulin tolerance tests were evaluated. The T-p animals showed no changes in somatic and biochemical parameters, while the NT + LPp group showed a greater abdominal circumference, hyperglycaemia, hypercholesterolaemia, glucose intolerance and lower plasma leptin. In the T + LPp animals, all of those alterations were reversed except for plasma leptin concentration. In conclusion, the effects of a perinatal low-protein diet on growth and development, glucose homeostasis and serum leptin concentration in the offspring were attenuated in pups from trained mothers.