Crystallization of PTP Domains.
Crystallization of PTP Domains.
复制标题
PTP 结构域的结晶。
DOI：
10.1007/978-1-4939-3746-2_10
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Levy C
中科院分区：
文献类型：
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作者：
Levy C
蛋白质结晶学是获取蛋白质三维结构原子分辨率信息的最有效方法。确定蛋白质晶体结构的一个重要步骤是从浓缩的纯化蛋白质样品中产生高质量的晶体。然后这些晶体被用来获得确定3D结构所需的X射线衍射数据，如果同源蛋白质的模型可用，则通过直接定相或分子替换来确定3D结构。在这里，我们描述了获得适合X射线衍射的晶体的主要途径和技术。我们包括如何评估和设计蛋白质结构、如何制备蛋氨酸衍生蛋白质、如何评估样品的稳定性和质量以及如何结晶和制备用于衍射实验的晶体的工具和指南。虽然总结了蛋白质结晶的一般策略，但讨论了这些策略在PTP结构域的结晶中的具体应用实例。
Protein crystallography is the most powerful method to obtain atomic resolution information on the three-dimensional structure of proteins. An essential step towards determining the crystallographic structure of a protein is to produce good quality crystals from a concentrated sample of purified protein. These crystals are then used to obtain X-ray diffraction data necessary to determine the 3D structure by direct phasing or molecular replacement if the model of a homologous protein is available. Here, we describe the main approaches and techniques to obtain suitable crystals for X-ray diffraction. We include tools and guidance on how to evaluate and design the protein construct, how to prepare Se-methionine derivatized protein, how to assess the stability and quality of the sample, and how to crystallize and prepare crystals for diffraction experiments. While general strategies for protein crystallization are summarized, specific examples of the application of these strategies to the crystallization of PTP domains are discussed.
影响因子：
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作者：
Phillips, Kevin;de la Pena, Andres Hernandez
通讯作者：
de la Pena, Andres Hernandez