Tetrahydroxystilbene glucoside attenuates MPP+-induced apoptosis in PC12 cells by inhibiting ROS generation and modulating JNK activation
Tetrahydroxystilbene glucoside attenuates MPP+-induced apoptosis in PC12 cells by inhibiting ROS generation and modulating JNK activation
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DOI：
10.1016/j.neulet.2010.07.027
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发表时间：
2010-10-08
影响因子：
2.5
通讯作者：
Kang, Xiaogang
中科院分区：
文献类型：
--
作者：
Li, Xiaobing;Li, Yan;Kang, Xiaogang
众所周知，氧化应激在帕金森病（PD）的进展中起着重要作用。以前的研究表明，从传统中药何首乌中提取的活性成分2，3，5，4 ′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷（TSG），具有显著的抗氧化和自由基清除活性。这是首次研究TSG对MPP+诱导的PC 12细胞凋亡的保护作用，并确定了潜在的机制。结果表明，TSG能明显降低MPP+对PC 12细胞的损伤程度，逆转细胞凋亡，且呈剂量依赖性。TSG的抗凋亡作用可能是通过抑制MPP+诱导的ROS增加和JNK磷酸化而实现的。综上所述，这些结果表明，TSG可能提供一个有用的治疗策略，用于治疗神经退行性疾病，如PD。(C)2010爱思唯尔爱尔兰有限公司版权所有。
It is known that oxidative stress plays a major role in the progression of Parkinson's disease (PD). Previous studies have suggested that 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside (TSG), an active component extracted from a traditional Chinese herb Polygonum multiflorum Thunb., has significant antioxidant and free radical-scavenging activities. This is the first study that investigated the protective effects of TSG against MPP+-induced apoptosis in PC12 cells and determined the underlying mechanism. The results showed that incubation of PC12 cells with TSG before exposing them to MPP+ could significantly decrease cell viability loss and reverse cell apoptosis in a dose-dependent manner. The anti-apoptotic effects of TSG were probably mediated via the inhibition of ROS generation and modulation of JNK activation because TSG blocked ROS increase and JNK phosphorylation induced by MPP+. Taken together, these results indicated that TSG may provide a useful therapeutic strategy for the treatment of neurodegenerative diseases such as PD. (C) 2010 Elsevier Ireland Ltd. All rights reserved.