New approach on evaluation of liposome release property with electric impedance measuring method
New approach on evaluation of liposome release property with electric impedance measuring method
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DOI：
10.1117/12.783960
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发表时间：
2007-12
期刊：
影响因子：
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通讯作者：
Guoming Chen;Zhongwei Jiang;M. Yoshimoto;Yafang Luo;Yunlong Wei
中科院分区：
文献类型：
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作者：
Guoming Chen;Zhongwei Jiang;M. Yoshimoto;Yafang Luo;Yunlong Wei
本研究的目的是建立一种通过检测脂质体溶液中的电阻抗变化来评价脂质体释放特性的新方法。制备了包裹钙黄蛋白的脂质体（钙黄蛋白脂质体）和去离子水（白脂质体），利用超声作为刺激使钙黄蛋白从脂质体中释放出来。测量超声处理前后去离子水中钙黄蛋白脂质体的阻抗变化，以及去离子水和钙黄蛋白溶液中白脂质体的阻抗变化进行比较。同时用荧光光谱法测定钙黄蛋白脂质体混悬液的释放度。结果表明，超声作用下白脂质体的阻抗变化较大，且与样品总离子浓度有关。用电阻抗计算的钙黄蛋白释放速率与荧光强度计算的结果吻合较好。结果表明，电阻抗测量法与荧光分析法一样具有较高的估计释放率的潜力，可用于荧光法无法评价的非荧光包封脂质体。
The purpose of this study is to develop a novel method for evaluation of the liposomes' release property by detecting the electric impedance change in the liposome solution. Liposomes encapsulating calcein (calcein-liposomes) and de-ionized water (white-liposomes) were prepared and ultrasound was employed as the stimulus in order to release the calcein from liposomes. The impedance change of calcein-liposomes in de-ionized water was measured before and after ultrasound treatment, as well as that of white-liposomes in de-ionized water and in calcein solutions for comparison. Besides, the release of calcein-liposome suspensions was evaluated simultaneously by spectrofluorometer. Results showed that the impedance of white-liposomes would change greatly when it was exposed to ultrasound and it was related to the total ion concentration of the sample. The release rate of calcein calculated with electric impedances showed a good agreement to that calculated with fluorescence intensities. It indicates that the electric impedance measuring method has high potential to estimate the release rate as the fluorescence analysis do, and might be used for non-fluorescent encapsulated liposomes which could not be evaluated by fluorescence method.