Social housing conditions and oxytocin and vasopressin receptors contribute to ethanol conditioned social preference in female mice.
Social housing conditions and oxytocin and vasopressin receptors contribute to ethanol conditioned social preference in female mice.
复制标题
DOI：
10.1016/j.physbeh.2015.08.018
复制
发表时间：
2015-11-01
影响因子：
2.9
通讯作者：
Levitt P
中科院分区：
文献类型：
--
作者：
Wood RI;Knoll AT;Levitt P
社会行为调节对酒精的反应。由于催产素（OXT）和抗利尿激素（AVP）有助于奖励社会行为，本研究利用遗传策略来确定OXT和AVP受体（OXTR, AVPR1a）是否对雌性小鼠表现出对乙醇的条件社会偏好至关重要。该研究在条件社会偏好（CSP）测试中比较了缺乏Oxtr或Apr1a基因的野生型（WT）和敲除型（KO）雌性。来自Het-Het杂交的KO雌性和WT雌性被成对饲养：KO， WT（KO）。来自Het-WT杂交的雌性WT被成对饲养：WT（WT）。实验小鼠接受2 g/kg的乙醇或生理盐水，在生理盐水后分别与雌性（CS -）和雌性（CS+）配对4次。配对后，测量与CS+和CS -雌性相处的时间。WT（WT）雌性对CS+雌性与乙醇配对表现出条件偏好，表现为更长的相互作用时间（p<0.05）。在两个KO系中，乙醇显著降低了与CS+雌性的相互作用（p<0.05），而对WT（KO）雌性的相互作用没有变化。在两个KO系中，习惯-不习惯对气味的反应不受影响，对催眠剂量的乙醇的反应也与WT小鼠相同。然而，与WT相比，KOOxtr雌性小鼠的焦虑程度有所降低（WT）。结果表明，Oxtr和Avpr1a是乙醇相关社会刺激条件效应所必需的。WT（ko）雌性缺乏CSP表明，出生后和断奶后的社会互动质量可能会调节正常社会反应的发育和表达。
Social behavior modulates response to alcohol. Because oxytocin (OXT) and vasopressin (AVP) contribute to rewarding social behavior, the present study utilized a genetic strategy to determine whether OXT and AVP receptors (OXTR, AVPR1a) are essential for female mice to demonstrate a conditioned social preference for ethanol. The study compared wild-type (WT) and knock-out (KO) females lacking either the Oxtr or Apr1a in a conditioned social preference (CSP) test. KO females and WT females from Het-Het crosses were pair-housed: KO, WT(ko). WT females from Het-WT crosses were pair-housed: WT(wt). Test mice received 2 g/kg ethanol or saline ip, and were paired four times each with one stimulus female (CS−) after saline, and with another female (CS+) following ethanol. After pairing, the time spent with CS+ and CS− females was measured. WT(wt) females showed conditioned preference for the CS+ female paired with ethanol, demonstrated by greater interaction time (p<0.05). In both KO lines, ethanol significantly reduced interaction with the CS+ female (p<0.05), and there was no change in interaction for WT(ko) females. Response to odors by habituation-dishabituation was unaffected in both KO lines, and the response to a hypnotic dose of ethanol also was the same as in WT mice. However, anxiety, measured as time on the open arms of the elevated plus maze, was reduced in KOOxtr females compared with WT(wt). The results suggest that Oxtr and Avpr1a are required for conditioned effects of an ethanol-associated social stimulus. The lack of CSP in WT(ko) females suggests that the quality of social interactions during postnatal and postweaning life may modulate development and expression of normal social responses.