ANTIOXIDANT, ANTIBACTERIAL AND CELL TOXICITY EFFECTS OF POLYPHENOLS FROMAHMEUR BOUAMER GRAPE SEED EXTRACTS
ANTIOXIDANT, ANTIBACTERIAL AND CELL TOXICITY EFFECTS OF POLYPHENOLS FROMAHMEUR BOUAMER GRAPE SEED EXTRACTS
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DOI：
10.4314/jfas.v9i1.24
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发表时间：
2017-01-01
影响因子：
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通讯作者：
Baaliouamer, A.
中科院分区：
文献类型：
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作者：
Ghouila, Z.;Laurent, S.;Baaliouamer, A.
在这项工作中，首次从葡萄籽提取物中获得了高浓度的总多酚和类黄酮（分别为 256.15 17.40 mg GAE/g(dm) 和 14.08 0.64 mg CE/g(dm)）。LC/MS 分析显示原花青素含量丰富。对于抗氧化、抗菌和抗真菌作用，葡萄籽提取物 (GSE) 的反应积极。在 100 μg/mL 浓度下，GSE 在治疗的前 24 小时内对 3T6 细胞产生约 3.88% 的中度毒性，而其延长作用至 48 小时则将该毒性降低至低于 0.5%。至于对肿瘤细胞系的抗增殖作用，在治疗24小时和48小时期间，HeLa和BCPAP细胞的细胞死亡分别为18.39%至23.79%和10.30%至20.37%。因此，可以考虑使用较低浓度的 GSE 作为抗增殖剂，同时又不会忽视其对健康细胞的有益作用。
In this work and for the first time, significant concentrations of total polyphenols and flavonoids from Vitis vinifera L. grape seed extracts were obtained (256.15 17.40 mg GAE/g(dm) and 14.08 0.64 mg CE/g(dm), respectively).The LC/MS analysis revealed richness in procyanidins. For antioxidant, antimicrobial and antifungal effects, the grape seed extract (GSE) responded positively. At 100 mu g/mL, GSE induced a moderate toxicity of the order of 3.88% on 3T6 cells at the first 24 hours of treatment, whereas, its prolonged effect to 48 hours reduced this toxicity to less than 0.5%. As for the anti-proliferative effect on tumoral cell lines, a cell death of 18.39% to 23.79% and 10.30% to 20.37% was registered respectively for HeLa and BCPAP cells during 24 and 48 hours of treatment. Consequently, it is possible to consider using GSE at lower concentrations as an anti-proliferative agent without losing sight of its benefic effect on healthy cells.