Transcriptome analysis of the response of silkworm to drastic changes in ambient temperature
Transcriptome analysis of the response of silkworm to drastic changes in ambient temperature
复制标题
家蚕对环境温度剧烈变化反应的转录组分析
DOI：
10.1007/s00253-018-9387-5
复制
发表时间：
2018-12-01
影响因子：
5
通讯作者：
Xia, Qingyou
中科院分区：
文献类型：
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作者：
Guo, Huizhen;Huang, Chunlin;Xia, Qingyou
家蚕是一种温热昆虫，对蚕丝生产具有重要的经济意义。环境温度的剧烈变化对养蚕有不利影响。然而，目前尚不清楚高温与家蚕疾病发生相关的原因以及家蚕对低温的反应，这是本研究的重点。以13°C （DZ-13）、25°C （DZ-25）和37°C （DZ-37）条件下的大枣家蚕进行RNA-seq分析。DZ-13和DZ-37分别有478个和194个差异表达基因上调，而DZ-13和DZ-37分别有49个和273个差异表达基因下调。8个基因共上调，其中7个基因与热休克蛋白（Hsps）有关，说明热休克蛋白在家蚕的高低温耐受性中起重要作用。基因本体分析显示DZ-13的发育过程下调。DZ-13中氧化磷酸化和胰岛素信号通路中的所有deg均上调。DZ-13中多种表皮蛋白和ATP合成相关基因表达上调，提示表皮增厚和ATP酶表达增加有助于家蚕抵御低温胁迫。在DZ-37中，bmreland bmserin -2等免疫相关基因下调，表明家蚕在高温冲击下抗性降低，对病原菌易感性。因此，家蚕耐热性的提高应与病原菌抗性的增强有关。
Bombyx moriis a poikilothermic insect and is economically important for silk production. Drastic changes in the ambient temperature have a negative impact on sericulture. However, the reason as to why high temperature is associated with the occurrence of diseases in silkworm and the response of silkworm to low temperature remain unclear and were the focus of the present study. Dazao silkworm exposed to 13 °C (DZ-13), 25 °C (DZ-25), and 37 °C (DZ-37) were used for RNA-seq analysis. There were 478 and 194 upregulated differentially expressed genes (DEGs) in DZ-13 and DZ-37 while 49 and 273 downregulated DEGs in DZ-13 and DZ-37, respectively. Eight DEGs were co-upregulated, in which seven genes were for heat shock proteins (Hsps), implying that Hsps play important roles in the tolerance of silkworm to high and low temperature. Gene ontology analysis revealed that the developmental process was downregulated in DZ-13. All the DEGs in the oxidative phosphorylation and insulin signaling pathways were upregulated in DZ-13. Several cuticular proteins and ATP synthesis-related genes were upregulated in DZ-13, suggesting that thickening of the cuticle and increase in the ATPase expression would help silkworms to protect themselves from low temperature-induced stress. Several immune-related genes, such asBmRelandBmSerpin-2, were downregulated in DZ-37, revealing that the resistance of silkworm is decreased under high temperature shock resulting in susceptibility to pathogens. Thus, the increase in the thermo-tolerance of silkworm should be related to the enhancement in the pathogen resistance.