Induction of amino acid transport in primary cultures of adult rat liver parenchymal cells by insulin.
Induction of amino acid transport in primary cultures of adult rat liver parenchymal cells by insulin.
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胰岛素诱导成年大鼠肝实质细胞原代培养物中的氨基酸转运。
DOI：
10.1016/s0021-9258(17)33492-0
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发表时间：
1976
期刊：
影响因子：
--
通讯作者：
F. Butcher
中科院分区：
文献类型：
--
作者：
R. Kletzien;M. Pariza;J. Becker;V. Potter;F. Butcher
氨基酸转运研究了原代培养的实质细胞分离成年大鼠肝脏胶原酶灌注技术，并保持在无血清培养基中的单层。通过测量非代谢氨基酸α-氨基异丁酸的摄取来测定氨基酸转运。大鼠肝实质细胞通过能量依赖性Na+需要系统转运α-氨基异丁酸，该系统显示Michaelis-Menten动力学。向培养的大鼠肝实质细胞中加入胰岛素导致α-氨基异丁酸的流入增加，这反映在α-氨基异丁酸转运的初始速率较高以及α-氨基异丁酸在随后时间点的蓄积增加。放线菌酮有效地阻止了增加，而放线菌素D的结果是模棱两可的。低至50 pM浓度的胰岛素可有效刺激α-氨基异丁酸转运，而在80 nM时观察到最大反应。
Amino acid transport was studied in primary cultures of parenchymal cells isolated from adult rat liver by a collagenase perfusion technique and maintained as a monolayer in a serum-free culture medium. Amino acid transport was assayed by measuring the uptake of the nonmetabolizable amino acid, alpha-aminoisobutyric acid. Rat liver parenchymal cells transported alpha-aminoisobutyric acid by an energy-dependent Na+-requiring system which displayed Michaelis-Menten kinetics. Addition of insulin to cultured rat liver parenchymal cells resulted in an increased influx of alpha-aminoisobutyric acid which was reflected in a higher initial rate of alpha-aminoisobutyric acid transport as well as an increased accumulation of alpha-aminoisobutyric acid at later time points. Cycloheximide effectively blocked the increase while results with actinomycin D were equivocal. Insulin at concentrations as low as 50 pM was effective in stimulating alpha-aminoisobutyric acid transport while the maximal response was observed at 80 nM.