Genetic evidence for the expression of ATP- and GTP-specific succinyl-CoA synthetases in multicellular eucaryotes
Genetic evidence for the expression of ATP- and GTP-specific succinyl-CoA synthetases in multicellular eucaryotes
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DOI：
10.1074/jbc.273.42.27580
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发表时间：
1998-10-16
影响因子：
4.8
通讯作者：
Lambeth, DO
中科院分区：
文献类型：
--
作者：
Johnson, JD;Mehus, JG;Lambeth, DO
鸽子中琥珀酰辅酶 A 合成酶的高度 ATP 和 GTP 特异性亚型包含相同的 α 亚基，但不同的 β 亚基（Johnson, J, D.、Muhonen, W. W. 和 Lambeth, D. O, (1998) J. Biol. Chem. 273, 27573-27579），成熟亚基的序列通过基于以下方法确定：逆转录聚合酶链反应。鸽子中 306 个残基的成熟 α 亚基与猪和大鼠中的同源物显示出 > 88% 的同一性。鸽子成熟 ATP 和 GTP 特异性 β 亚基（分别为 A-β 和 G-β）的序列有 54% 相同。这些序列用于鉴定人和小鼠的表达序列标签，其分别与G-β和A-β高度同源。小鼠和人类成熟 A-β 和 G-β 的序列已完成并通过聚合酶链式反应进行验证。还获得了猪的A-β序列。哺乳动物 A-β 序列彼此显示 >89% 的同一性； G-β 序列类似地相关，然而，A-P 和 G-β 序列的成对比较表明
Highly ATP- and GTP-specific isoforms of succinyl-CoA synthetase in pigeon incorporate the same alpha-subunit, but different beta-subunits (Johnson, J, D., Muhonen, W. W., and Lambeth, D. O, (1998) J. Biol. Chem. 273, 27573-27579), The sequences of the mature subunits were determined by methods based on reverse transcription-polymerase chain reaction. The 306-residue mature alpha-subunit in pigeon shows >88% identity to its homologues in pig and rat. The sequences of the mature ATP- and GTP-specific beta-subunits (A-beta and G-beta, respectively) in pigeon are 54% identical. These sequences were used to identify expressed sequence tags for human and mouse that were highly homologous to G-beta and A-beta, respectively. The sequences for mature A-beta and G-beta in mouse and human were completed and verified by polymerase chain reaction. The sequence of A-beta in pig was also obtained. The mammalian A-beta sequences show >89% identity to each other; the G-beta sequences are similarly related, However, pairwise comparisons of the A-P and G-beta sequences revealed