Expressional and functional comparisons of two general odorant binding proteins in Agrotis ipsilon
Expressional and functional comparisons of two general odorant binding proteins in Agrotis ipsilon
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小地老虎中两种通用气味结合蛋白的表达和功能比较
DOI：
10.1016/j.ibmb.2018.05.003
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发表时间：
2018-07-01
影响因子：
3.8
通讯作者：
Gu, Shao-Hua
中科院分区：
文献类型：
--
作者：
Huang, Guang-Zhen;Liu, Jing-Tao;Gu, Shao-Hua
长期以来，昆虫一般气味结合蛋白(GOBP)被认为与寄主植物的挥发性物质结合并运输到嗅神经元树突膜上的嗅觉受体上。最近的研究表明，它们还可以结合雌性性信息素。本研究从小地老虎成虫触角中克隆了两个GOBP基因AipsGOBP1和AipsGOBP2。组织表达谱表明，它们都是触角特有的，并且在雌性触角中比在雄性触角中更丰富。时间表达谱显示，AipsGOBP1和AipsGOBP2在成虫羽化前3d开始在触角表达，成虫羽化后3d和4d分别达到最高表达水平。交配增加了它们在雌性触角中的表达，但减少了它们在雄性触角中的表达。原位杂交和免疫定位结果表明，AipsGOBP1和AipsGOBP2在两性基本感受器和毛感感受器中均有表达和共存。AipsGOBP2与两种主要的性信息素组分Z7-12：Ac和Z9-14：Ac以及4种植物挥发物顺-3-己烯-L醇、油酸、邻苯二甲酸二丁酯和吉丁香烯具有较高的体外结合亲和力，而AipsGOBP1则与5种性信息素和6种植物挥发物表现出中等的结合亲和力。AipsGOBP2还表现出比AipsGOBP1更宽的配基结合谱和更高的配基结合亲和力，与鳞翅目物种的醛和醇性信息素结合。综上所述，我们的结果表明，AipsGOBP2在结合性信息素和寄主植物挥发物方面可能比AipsGOBP1发挥更大的作用。
Insect general odorant binding proteins (GOBPs) have been long thought to bind and transport host plant volatiles to the olfactory receptors on the dendrite membrane of the olfactory neurons. Recent studies indicate that they can also bind female sex pheromones. In present study, two GOBP genes, AipsGOBP1 and AipsGOBP2 were cloned from the adult antennae of Agrotis ipsilon. Tissue expression profiles indicated that both of them are antennae-specific and more abundant in the female antennae than in the male antennae. Temporal expression profiles showed that both AipsGOBP1 and AipsGOBP2 began to express in antennae 3 days prior to adult emergence from pupae, and reached their highest expression level 3 and 4 days after adult emergence, respectively. Mating increased their expression in the female antennae but reduced their expression in the male antennae. In situ hybridization and immunolocalization demonstrated that both AipsGOBP1 and AipsGOBP2 are expressed and co-localized in sensilla basiconica and sensilla trichodea of both sexes. AipsGOBP2 exhibited a high binding affinity in vitro with the two major sex pheromone components Z7-12:Ac and Z9-14:Ac and the four plant volatiles cis-3-hexen-l-ol, oleic acid, dibutyl phthalate and jI-caryophyllene with Ki values less than 5 mu M. AipsGOBP1, on the other hand, showed medium binding affinities with the five A. ipsilon sex pheromones and six plant volatiles. AipsGOBP2 also showed a broader ligand-binding spectrum and a greater ligand-binding affinity than AipsGOBP1 with the tested aldehyde and alcohol sex pheromones of Lepidoptera species. Taken together, our results indicate that AipsGOBP2 may play greater roles than AipsGOBP1 does in binding sex pheromones and host plant volatiles.