Significance of CD99 expression in T-lineage acute lymphoblastic leukemia
Significance of CD99 expression in T-lineage acute lymphoblastic leukemia
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DOI：
10.3233/cbm-160608
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发表时间：
2016-01-01
影响因子：
3.1
通讯作者：
Fathalla, Lamiaa A.
中科院分区：
文献类型：
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作者：
Enein, Azza A. Aboul;Rahman, Hala A. Abdel;Fathalla, Lamiaa A.
背景：CD 99最早是作为T急性淋巴细胞白血病细胞表面的抗原分离出来的。研究目的：检测CD 99在白血病和正常T细胞上的表达模式，并评估其作为诊断和监测T-ALL病例的工具的可能性。方法：采用流式细胞术检测62例初治TALL患者外周血淋巴细胞中CD 99的表达水平。在治疗后第15天和第42天随访患者是否存在微小残留病变。结果：CD 99在所有T-ALL患者中均有表达，中位表达率为58.5%，明显高于对照组的1.38%（P < 0.001）。在治疗后第42天，100%的初始CD 99表达50%的随访患者没有微小残留病变（P = 0.03）。结论：CD 99表达对T-ALL患者1年生存率无显著影响（P = 0.82），可作为T-ALL患者治疗后随访中微小残留病诊断和监测的补充。需要进一步的研究来证实这些发现。
BACKGROUND: CD99 was first isolated as an antigen on the T acute lymphoblastic leukemia cells. It has been shown to participate in T cell adhesion and is widely expressed on a variety of hematopoietic and non-hematopoietic cell types.AIM OF WORK: Detection of the expression pattern of CD99 on leukemic and normal T cells and assessing the possibility of its use as a tool for the diagnosis and monitoring of T-ALL cases.METHODOLOGY: We used flow cytometry technique to determine the expression level of CD99 in 62 newly diagnosed TALL patients. Patients were followed up for the presence of minimal residual disease on day 15 and day 42 post-therapy. 20 age and sex matched healthy controls were enrolled in our study.RESULTS: CD99 was expressed in all T-ALL patients, with a higher median expression level when compared to controls (58.5% versus 1.38%, p < 0.001). On day 42 post-therapy, 100% of follow up patients who had initial CD99 expression 50% had no minimal residual disease (P = 0.03). There was no significant influence of CD99 expression on the 1-year overall survival probability (P = 0.82).CONCLUSION: CD99 could be used to complement current strategy relying on TdT for diagnosis and monitoring of minimal residual disease during the post-therapy follow up of T-ALL patients. Further studies are needed to confirm these findings.