Identification, classification and phylogeny of the Aspergillus section Nigri inferred from mitochondrial cytochrome b gene.
Identification, classification and phylogeny of the Aspergillus section Nigri inferred from mitochondrial cytochrome b gene.
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从线粒体细胞色素b基因推断黑曲霉组的鉴定、分类和系统发育。
DOI：
10.1111/j.1574-6968.2001.tb10722.x
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发表时间：
2001
影响因子：
2.1
通讯作者：
K. Nishimura
中科院分区：
文献类型：
--
作者：
K. Yokoyama;L. Wang;M. Miyaji;K. Nishimura
采用聚合酶链反应（PCR）技术，对黑曲霉组12个种32个菌株的线粒体细胞色素B基因进行了扩增和序列测定。利用402个核苷酸特征，构建了基于核苷酸和氨基酸的系统发育树，并对物种间的遗传分化进行了评价。根据402 bp的核苷酸序列和133个氨基酸的序列分析，菌株分为11个DNA型和5个氨基酸型。尼日尔曲霉和泡盛曲霉显示不同的氨基酸序列。A.尼日尔分支包括A.尼日尔湾尼日尔和无花果曲霉。A. awamori分支包括A.泡盛曲霉（Aspergillus awamori）、凤凰曲霉（Aspergillus phoenoptera）、粉曲霉（Aspergillus powerfulentus）、塔宾曲霉（Aspergillus tubingensis）、臭曲霉（Aspergillus foetidus）和两个变种。尼日尔湾纳努斯和瓦尔。中间体A.尼日尔将被重新归类。A.重新鉴定了一株黑曲霉和一株炭曲霉。
The partial mitochondrial cytochrome b gene from 32 strains of 12 species belonging to Aspergillus section Nigri was amplified by the polymerase chain reaction and sequenced directly. Using 402 nucleotide characters, nucleotide-based and amino acid-based phylogenetic trees were inferred and the genetic divergence among the species was evaluated. Based on analyses of the 402-bp nucleotide and 133-amino acid sequences, strains were divided into 11 DNA types and five amino acid types. Aspergillus niger and Aspergillus awamori showed different amino acid sequences. A. niger clade included A. niger var. niger and Aspergillus ficuum. A. awamori clade included A. awamori, Aspergillus phoenicis, Aspergillus pulverulentus, Aspergillus tubingensis, Aspergillus foetidus, and two varieties of A. niger, var. nanus and var. intermedius. Two varieties of A. niger will be reclassified. One strain of A. phoenicis and one strain of Aspergillus carbonarius were reidentified.