Detection of a High-Turnover Serotonin Circuit in the Mouse Brain Using Mass Spectrometry Imaging
Detection of a High-Turnover Serotonin Circuit in the Mouse Brain Using Mass Spectrometry Imaging
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DOI：
10.1016/j.isci.2019.09.036
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发表时间：
2019-10-25
期刊：
影响因子：
5.8
通讯作者：
Sugiura, Yuki
中科院分区：
文献类型：
--
作者：
Sugiyama, Eiji;Guerrini, Matteo M.;Sugiura, Yuki
单胺类神经递质由调节行为、运动和认知功能的特殊神经元释放。虽然单胺能神经元在大脑中的定位是众所周知的，但单胺的分布和动力学仍然不清楚。在这里，我们使用质谱仪成像(MSI)生成了小鼠脑内5-羟色胺(5-羟色胺)、多巴胺(DA)和去甲肾上腺素(NE)水平的图谱。我们发现了几个同时富含5-羟色胺和儿茶酚胺(DA或NE)的核团，并确定了丘脑室旁核(PVT)，其中5-羟色胺和去甲肾上腺素共存。对急性色氨酸耗竭和体内注入同位素标记色氨酸后5-羟色胺波动的分析表明，中缝核群、PVT和杏仁核之间存在密切的动力学联系，而其他核团则没有。我们的发现暗示存在一个高度动态的5-羟色胺介导的中缝至PVT通路，该通路可能在大脑单胺系统中发挥作用。
Monoamine neurotransmitters are released by specialized neurons regulating behavioral, motor, and cognitive functions. Although the localization of monoaminergic neurons in the brain is well known, the distribution and kinetics of monoamines remain unclear. Here, we generated a murine brain atlas of serotonin (5-HT), dopamine (DA), and norepinephrine (NE) levels using mass spectrometry imaging (MSI). We found several nuclei rich in both 5-HT and a catecholamine (DA or NE) and identified the paraventricular nucleus of the thalamus (PVT), where 5-HT and NE are co-localized. The analysis of 5-HT fluctuations in response to acute tryptophan depletion and infusion of isotope-labeled tryptophan in vivo revealed a close kinetic association between the raphe nuclei, PVT, and amygdala but not the other nuclei. Our findings imply the existence of a highly dynamic 5-HT-mediated raphe to PVT pathway that likely plays a role in the brain monoamine system.