Are peripheral blood cells from patients with Alzheimer disease more sensitive to apoptotic stimuli?
Are peripheral blood cells from patients with Alzheimer disease more sensitive to apoptotic stimuli?
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DOI：
10.1097/00002093-200207000-00005
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发表时间：
2002-07-01
影响因子：
2.1
通讯作者：
Bessler, T
中科院分区：
文献类型：
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作者：
Bergman, M;Salman, H;Bessler, T
阿尔茨海默病患者对感染的易感性增加的原因之一可能是其外周白细胞的凋亡增加。如果是这种情况，则细胞凋亡增强可能是由于患者血清中的成分或细胞对细胞凋亡刺激的敏感性增加所致。为了检验这种可能性，我们将 12 名阿尔茨海默病患者的外周血单核细胞 (PBMC) 中凋亡细胞的百分比与 12 名年龄匹配的非痴呆症患者和 12 名中年健康对照受试者的百分比进行了比较。此外，还检测了三组受试者血清对健康对照受试者外周b1cod细胞凋亡、白细胞介素(IL)-1β、IL-2、IL-6、IL-10和肿瘤坏死因子(TNF)α产生的影响。结果发现，阿尔茨海默病患者的PBMC凋亡百分比高于其余两组。然而，将对照细胞与阿尔茨海默病患者和非痴呆老年人的血清一起孵育并不影响凋亡细胞的数量。阿尔茨海默病患者和非痴呆老年受试者的血清导致中年对照受试者的 PBMC 产生 IL-2 增加和 IL-10 产生减少，但不影响 IL-1β、IL-6 和 TNFα 分泌，表明与衰老相关的免疫系统发生变化。
One of the reasons for the increased susceptibility to infections in patients with Alzheimer disease may be enhanced apoptotic death of their peripheral leukocytes. If this is the case, the enhanced apoptosis may be due to components in the patients' sera or to an increased sensitivity of the cells to apoptotic Stimuli. To examine this possibility, the percentage of apoptotic cells in the peripheral blood mononuclear cells (PBMC) from 12 patients with Alzheimer disease was compared with that of 12 age-matched non-demented persons and 12 middle-aged healthy control subjects. In addition, the effect of sera from subjects in the three groups on the apoptosis, interleukin (IL)-1beta, IL-2, IL-6, IL-10, and tumor necrosis factor (TNF)alpha production by peripheral b1cod cells from healthy control subjects was examined. It was found that the percentage of apoptotic PBMC from patients with Alzheimer disease was higher than that from the remaining two groups. However, incubation of control cells with sera from patients with Alzheimer disease and non-demented elderly persons did not affect the number of apoptotic cells. Sera from patients with Alzheimer disease and non-demented elderly subjects caused an increase in IL-2 and a decrease in IL-10 production by PBMC from middle-aged control subjects but did not affect IL-1beta, IL-6, and TNFalpha secretion, indicating alterations of the immune system related to aging.