Integrated proteogenomic approach identifying a protein signature of COPD and a new splice variant of SORBS1
Integrated proteogenomic approach identifying a protein signature of COPD and a new splice variant of SORBS1
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DOI：
10.1136/thoraxjnl-2019-213200
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发表时间：
2020-02-01
期刊：
影响因子：
10
通讯作者：
Horvatovich, Peter
中科院分区：
文献类型：
--
作者：
Brandsma, Corry-Anke;Guryev, Victor;Horvatovich, Peter
慢性阻塞性肺疾病（COPD）中基因组改变转化为蛋白质变化在很大程度上尚未探索。使用COPD和对照肺组织的整合蛋白质组学和RNA测序分析，我们确定了COPD中以细胞外基质变化为特征的蛋白质特征和SUMO 2的潜在调节作用。此外，我们确定了61差异表达的新的，非参考，肽在COPD与对照肺相比。这包括编码SORBS1的新剪接变体的两种肽，与对照肺相比，COPD中的转录本使用率更高。这些探索性发现和整合蛋白基因组学方法为进一步阐明COPD的病理学开辟了新的途径。
Translation of genomic alterations to protein changes in chronic obstructive pulmonary disease (COPD) is largely unexplored. Using integrated proteomic and RNA sequencing analysis of COPD and control lung tissues, we identified a protein signature in COPD characterised by extracellular matrix changes and a potential regulatory role for SUMO2. Furthermore, we identified 61 differentially expressed novel, non-reference, peptides in COPD compared with control lungs. This included two peptides encoding for a new splice variant of SORBS1, of which the transcript usage was higher in COPD compared with control lungs. These explorative findings and integrative proteogenomic approach open new avenues to further unravel the pathology of COPD.