The small proteoglycans decorin and biglycan in human articular cartilage of late-stage osteoarthritis
The small proteoglycans decorin and biglycan in human articular cartilage of late-stage osteoarthritis
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DOI：
10.1053/joca.2001.0420
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发表时间：
2001-10-01
影响因子：
7
通讯作者：
Miosge, N
中科院分区：
文献类型：
--
作者：
Bock, HC;Michaeli, P;Miosge, N
目的：透明透明软骨代谢的障碍在退行性关节疾病的病理中起着至关重要的作用。我们研究了转录表表达，蛋白质合成与基质 - 组织蛋白聚糖蛋白的蛋白聚糖蛋白和在后期骨关节炎人类关节软骨后期的细胞内和细胞外隔室内的超微结构定位之间的关系。从膝关节毗邻区域和主要缺陷的区域。研究了晚期骨关节炎的患者。在光和电子显微镜水平上分别进行原位杂交和免疫原性组织化学。分析：超大型结构，确定了三种主要的软骨细胞类型。 Decorin和Biglycan的最高水平是由已知可以合成1型胶原蛋白的细长分泌的2型细胞产生的。高水平mRNA的细胞还翻译了在细胞外室中发现的相应蛋白质。在与主要缺陷相邻的组织区域中看到了Decorin和Biglycan的最高生产率。结论：结果表明，在骨关节炎的后期阶段，用于Decorin和Biglycan的转录和翻译水平是上调的，可能是为了努力。补偿总体蛋白聚糖损失，这是该疾病阶段的特征。 （c）2001年骨关节炎研究协会国际。
Objective: Disturbances in proteoglycan metabolism of hyaline cartilage play an essential role in the pathology of degenerative joint disease. We investigated the relation between transcript expression, protein synthesis and the ultrastructural localization of the matrix-organizing proteoglycans decorin and biglycan within intra- and extracellular compartments of late-stage osteoarthritic human articular cartilage.Methods: Human cartilage samples of a macroscopically intact area, the adjoining area and an area of the main defect from knee joints of 10 patients with late stage osteoarthritis were investigated. In situ hybridization and immunogold histochemistry were carried out separately and in combination at the light and electron microscopic level.Results: Ultrastructurally, three main chondrocyte types were identified. The highest levels of mRNA of decorin and biglycan were produced by elongated secretory type 2 cells, already known to synthesize type 1 collagen. Cells with high levels of mRNA also translated the corresponding proteins to be found in the extracellular compartment. The highest production rate of decorin and biglycan was seen in the tissue area adjoining the main defect.Conclusion: The results indicate that at late stages of osteoarthritis the levels of transcription and translation for decorin and biglycan are up-regulated, probably in an effort to compensate for the general proteoglycan loss, characteristic of this disease stage. (C) 2001 OsteoArthritis Research Society International.