EXPRESSION OF JUN, KROX, AND CREB TRANSCRIPTION FACTORS IN GOLDFISH AND RAT RETINAL GANGLION-CELLS FOLLOWING OPTIC-NERVE LESION IS RELATED TO AXONAL SPROUTING
EXPRESSION OF JUN, KROX, AND CREB TRANSCRIPTION FACTORS IN GOLDFISH AND RAT RETINAL GANGLION-CELLS FOLLOWING OPTIC-NERVE LESION IS RELATED TO AXONAL SPROUTING
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DOI：
10.1002/neu.480240410
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发表时间：
1993-04-01
期刊：
影响因子：
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通讯作者：
ZIMMERMANN, M
中科院分区：
文献类型：
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作者：
HERDEGEN, T;BASTMEYER, M;ZIMMERMANN, M
采用免疫细胞化学方法观察视网膜神经节细胞（RGCs）中转录因子c-JUN、JUN B、JUN D、c-FOS、FOS B、KROX-24和CREB的表达。免疫反应性（IR）在金鱼中随访了350天，在大鼠中随访了22天。在未经处理的金鱼和大鼠的RGCs中，所有JUN，FOS和KROX蛋白均不存在，而CREB组成型表达。金鱼视神经切断后24 h，视网膜中央部少数RGCs出现JUN样免疫反应（JUN-IR），5 d内达到高峰，30 d后下降，50 d后达到半高峰。 在100至200天之间，JUN-IR仅在少数RGC中可见，并且在350天后完全不存在。抗c-JUN、JUN B的特异性抗体 而JUN D没有明显的免疫反应信号。因此，我们无法确定JUN家族的哪个成员促成了JUN-IR。CREB的表达在5天后下降。CREB标记的RGCs的数量减少（不显著），标记的强度逐渐消失。50天后，CREB-IR恢复到基础水平。c-FOS、FOS B和KROX-24在视神经切断后的RGCs中未检测到。在大鼠视神经挤压后，c-JUN、JUN D和KROX-24在24 h后出现在大量RGCs中，在5天后表达最大，在8天后强烈下降。c-JUN和KROX-24在22天后完全不存在，而JUN D仍然存在于少数大鼠RGC中。CREB标记的RGCs的数量在5天后减少，并且在22天后减少了50%。大鼠视神经夹伤后RGCs未见JUN B、c-FOS、FOS B表达。我们的数据表明，CREB的减少和增加的JUN和KROX-24转录因子之前和平行的从头蛋白质合成的改变和轴突发芽，这是长期持续的金鱼和短暂的大鼠。
Goldfish and rat optic nerves were cut and crushed, respectively, and the expression of the transcription factor proteins c-JUN, JUN B, JUN D, c-FOS, FOS B, KROX-24, and CREB was investigated in retinal ganglion cells (RGCs) by immunocytochemistry. Immunoreactivities (IRs) were followed up to 350 days in the goldfish and up to 22 days in the rat. In RGCs of untreated goldfish and rats, all JUN, FOS, and KROX proteins were absent whereas CREB was constitutively expressed. After optic nerve cut in goldfish, a JUN-like immunoreactivity (JUN-IR) appeared in a small number of RGCs of central retina after 24 h, reached a maximum within 5 days, declined after 30 days, and was on a half-maximal level after 50 days. Between 100 and 200 days, JUN-IR was only visible in a few RGCs and was completely absent after 350 days. Specific antibodies against c-JUN, JUN B and JUN D gave no distinct immunoreactive signal. Thus, we could not determine which member of the JUN family contributed to the JUN-IR. The expression of CREB declined after 5 days. The number of CREB-labeled RGCs was reduced (not significant) and the intensity of labeling faded out. After 50 days, CREB-IR had returned to basal level. c-FOS, FOS B, and KROX-24 could not be detected in goldfish RGCs following optic nerve cut. After optic nerve crush in the rat, c-JUN, JUN D, and KROX-24 appeared in a substantial number of RGCs after 24 h, had a maximal expression after 5 days, and strongly declined after 8 days. c-JUN and KROX-24 were completely absent after 22 days whereas JUN D was still present in a few rat RGCs. The number of CREB-labeled RGCs decreased after 5 days and had declined by 50% after 22 days. Expression of JUN B, c-FOS, FOS B could not be detected in rat RGCs after optic nerve crush. Our data demonstrate that the decrease of CREB and the increase of JUN and KROX-24 transcription factors precedes and parallels both the alteration of de novo protein synthesis and the axonal sprouting, which are long lasting in goldfish and transient in rat.