Label-Free Fluorescent Determination of Sunset Yellow in Soft Drinks Based on an Indicator-Displacement Assay
Label-Free Fluorescent Determination of Sunset Yellow in Soft Drinks Based on an Indicator-Displacement Assay
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DOI：
10.1155/2018/6302345
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发表时间：
2018-02
影响因子：
3.3
通讯作者：
Shiliang Wu;Yanqiong Zhang;Long Yang;Can-Peng Li
中科院分区：
文献类型：
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作者：
Shiliang Wu;Yanqiong Zhang;Long Yang;Can-Peng Li
本文报道了一种基于葫芦[7]uril （CB7）与信号探针/靶分子之间的竞争主客相互作用的日落黄（SY）荧光传感平台。选择木犀草素/表没食子儿茶素没食子酸酯（EGCG）和SY分别作为探测分子和靶分子。木犀草素或EGCG进入CB7宿主后，其荧光明显增强。然而，当木犀草素·CB7或EGCG·CB7复合物中存在SY时，会导致荧光显著降低。这是由于CB7/SY （M−1）的结合常数大于CB7/木犀草素（M−1）或CB7/EGCG （M−1）。在0.5 ~ 50.0 μM和2.0 ~ 50.0 μM范围内，CB7/木犀草素复合物和CB7/EGCG复合物的荧光强度随SY浓度的增加呈线性下降。该方法检出限分别为0.12 μM和0.45 μM，回收率为96.3% ~ 103.8%。这种竞争模式为在食品安全方面的潜在应用提供了有前途的荧光分析策略。
This work reported a fluorescence sensing platform for Sunset Yellow (SY) determination based on competitive host-guest interaction between cucurbit[7]uril (CB7) and signal probe/target molecules. Luteolin/epigallocatechin gallate (EGCG) and SY were selected as the probe and target molecules, respectively. When luteolin or EGCG entered the CB7 host, its fluorescence significantly improved. However, upon the presence of SY in the performed luteolin·CB7 or EGCG·CB7 complex, this led to a remarkable decrease in fluorescence. This result was due to the fact that the binding constant of CB7/SY ( M−1) was greater than that of CB7/luteolin ( M−1) or CB7/EGCG ( M−1). The fluorescence intensities of CB7/luteolin and CB7/EGCG complexes decreased linearly with increased SY concentration ranges of 0.5–50.0 and 2.0–50.0 μM. The proposed method had detection limits of 0.12 and 0.45 μM and was successfully used to determine SY samples with good recoveries ranging from 96.3% to 103.8%. This competitive mode provided a promising fluorescence assay strategy for potential applications in food safety.