Real-time PCR for determining capsular serotypes of Haemophilus influenzae
Real-time PCR for determining capsular serotypes of Haemophilus influenzae
复制标题
DOI：
10.1128/jcm.00102-07
复制
发表时间：
2007-07-01
影响因子：
9.4
通讯作者：
Crokaert, Francoise
中科院分区：
文献类型：
--
作者：
Maaroufi, Younes;De Bruyne, Jean-Marc;Crokaert, Francoise
一种靶向流感嗜血杆菌所有六个荚膜位点（即，血清型a至f），并与先前发表的使用131 H的定性PCR测定进行比较。流感临床分离株。两次检测的一致性为98.5%。检测荚膜型特异性参考株的敏感性。a ~ c型流感病毒（10（1）CFU/PCR）比e型流感病毒（10（3）CFU/PCR）和d、f型流感病毒（10（4）CFU/PCR）高，并且获得了更宽的动态范围（5 ~ 8 log（10）单位）。与呼吸道常见分离细菌无交叉反应。我们表明，这两种PCR检测方法比玻片凝集血清分型更可靠。基于实时PCR的检测方法似乎是H.流感侵袭性感染。
A two-step real-time PCR assay targeting all six capsulation loci of Haemophilus influenzae (i.e., serotypes a to f) was developed and compared with a previously published qualitative PCR assay by using 131 H. influenzae clinical isolates. There was a 98.5% concordance between the two tests. The sensitivity of detection of capsular type-specific reference strains of H. influenzae a to c (10(1) CFU/PCR) was higher than that for type e (10(3) CFU/PCR) and types d and f (10(4) CFU/PCR), and a broader dynamic range was obtained (5 to 8 log(10) units). No cross-reaction was observed with bacteria commonly isolated from the respiratory tract. We showed that both PCR assays are more reliable than slide agglutination serotyping. The real-time PCR-based assay seems to be an alternative of choice for the epidemiological follow-up of H. influenzae invasive infections.