Biomarkers associated with disease severity in allergic and nonallergic asthma
Biomarkers associated with disease severity in allergic and nonallergic asthma
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DOI：
10.1016/j.molimm.2016.12.012
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发表时间：
2017-02-01
影响因子：
3.6
通讯作者：
Cardaba, Blanca
中科院分区：
文献类型：
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作者：
Baos, Selene;Calzada, David;Cardaba, Blanca
哮喘是一种复杂的慢性呼吸系统疾病，具有广泛的临床症状。高通量技术的使用产生了大量需要验证的数据。在这项工作中，目的是验证外周血样本中与哮喘疾病类型相关的分子生物标志物，并确定它们与疾病严重程度的关系。为此，采用qRT-PCR分析了30名健康对照（HC）、30名非过敏性哮喘（NA）、30名过敏性哮喘（AA）和14名过敏（鼻炎）但无哮喘（AR）患者的94个先前描述的基因。采用TRIzol法从外周血单核细胞（PBMCs）中提取RNA。数据归一化后，进行主成分分析（PCA），并采用多种方法检测差异基因表达。相关性由RQ（相对量化）和校正P值(
Asthma is a complex, chronic respiratory disease with a wide clinical spectrum. Use of high-throughput technologies has generated a great deal of data that require validation. In this work the objective was to validate molecular biomarkers related to asthmatic disease types in peripheral blood samples and define their relationship with disease severity. With this purpose, ninety-four previously described genes were analyzed by qRT-PCR in 30 healthy control (HC) subjects, 30 patients with nonallergic asthma (NA), 30 with allergic asthma (AA), and 14 patients with,allergy (rhinitis) but without asthma (AR). RNA was extracted from peripheral blood mononuclear cells (PBMCs) using the TRIzol method. After data normalization, principal component analysis (PCA) was performed, and multiple approaches were used to test for differential gene expression. Relevance was defined by RQ (relative quantification) and corrected P value (