Role of TGF-β Signaling in Coupling Bone Remodeling
Role of TGF-β Signaling in Coupling Bone Remodeling
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DOI：
10.1007/978-1-4939-2966-5_18
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发表时间：
2016-01-01
期刊：
影响因子：
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通讯作者：
Cao, Xu
中科院分区：
文献类型：
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作者：
Crane, Janet L.;Xian, Lingling;Cao, Xu
转化生长因子-β（TGF - beta）信号在骨重塑的时空调节中起着关键作用。在破骨细胞骨吸收过程中，TGF - beta从骨基质中释放并被激活。活化的TGF - beta通过SMAD信号通路将间充质干细胞募集到骨吸收陷窝。间充质干细胞进行成骨细胞分化，并在吸收陷窝中沉积新骨，从而维持骨骼的结构完整性。因此，TGF - beta信号在耦合过程中起关键作用，TGF - beta信号通路的中断会导致骨骼完整性的丧失。本章介绍了如何定量骨基质中的TGF - beta以及评估其在体外和体内间充质干细胞迁移中的作用的方法。
TGF-beta signaling plays a key role in the temporal and spatial regulation of bone remodeling. During osteoclast bone resorption, TGF-beta is released from the bone matrix and activated. Active TGF-beta recruits mesenchymal stem cells to the bone resorption pit through the SMAD signaling pathway. Mesenchymal stem cells undergo osteoblast differentiation and deposit new bone filling in the resorption pit and maintaining the structural integrity of the skeleton. Thus, TGF-beta signaling plays a key role in the coupling process and disruptions to the TGF-beta signaling pathway lead to loss of skeletal integrity. This chapter describes methods on how to quantitate bone matrix TGF-beta and assess its role in mesenchymal stem cell migration both in vitro and in vivo.