Aluminum tolerance associated with enhancement of plasma membrane H+-ATPase in the root apex of soybean
Aluminum tolerance associated with enhancement of plasma membrane H+-ATPase in the root apex of soybean
复制标题
DOI：
10.1111/j.1747-0765.2009.00437.x
复制
发表时间：
2010-02
影响因子：
2
通讯作者：
Yu-Seon Kim;W. Park;H. Nian;Takayuki Sasaki;B. Ezaki;Y. Jang;G. Chung;H. Bae;Sung-ju Ahn
中科院分区：
文献类型：
--
作者：
Yu-Seon Kim;W. Park;H. Nian;Takayuki Sasaki;B. Ezaki;Y. Jang;G. Chung;H. Bae;Sung-ju Ahn
摘要对17个大豆品种进行了铝敏感性的筛选。选择耐铝品种Sowon和铝敏感品种Poongsan，通过简单的生长测定进行进一步研究。不同铝浓度(0、25、50、75或100μ·L~(-1))和暴露时间(0、3、6、12或24 h)的差异不同，但盆山品种的铝诱导的根生长抑制显著高于索旺品种。损伤主要发生在根尖。使用印度墨水进行的高灵敏度生长测量表明，中心延伸区位于距根尖2-3 mm处。50μ摩尔的L-1铝处理12h后，索旺品种根尖0~5 mm处的铝含量低于蓬桑品种，而且其柠檬酸分泌量是索旺品种的两倍多。铝胁迫24 h后，两个品种根尖(0-5 mm)质膜H+-ATPase的蛋白质产量均升高。但在铝处理的两个品种中，这种活性都有所下降，其中庞山品种受到的影响更严重。我们认为铝诱导的生长抑制与质膜H+-ATPase活性的变化有关，而质膜H+-ATPase活性的变化与根尖柠檬酸的分泌有关。
Abstract Seventeen soybean cultivars were screened to discern differences in aluminum (Al) sensitivity. The Sowon (Al-tolerant) and Poongsan (Al-sensitive) cultivars were selected for further study by simple growth measurement. Aluminum-induced root growth inhibition was significantly higher in the Poongsan cultivar than in the Sowon cultivar, although the differences depended on the Al concentration (0, 25, 50, 75 or 100 μmol L–1) and the amount of exposure (0, 3, 6, 12 or 24 h). Damage occurred preferentially in the root apex. High-sensitivity growth measurements using India ink implicated the central elongation zone located 2–3 mm from the root apex. The Al content was lower 0–5 mm from the root apices in the Sowon cultivar than in the apices of the Poongsan cultivar when exposed to 50 μmol L–1 Al for 12 h. Furthermore, the citric acid exudation rate was more than twofold higher in the Sowon cultivar. Protein production of plasma membrane (PM) H+-ATPase from the root apices (0–5 mm) was upregulated in the presence of Al for 24 h in both cultivars. This activity, however, decreased in both cultivars treated with Al and the Poongsan cultivar was more severely affected. We propose that Al-induced growth inhibition is correlated with changes in PM H+-ATPase activity, which is linked to the exudation of citric acid in the root apex.