A quantitative microwell assay for chondrocyte cell adhesion.
A quantitative microwell assay for chondrocyte cell adhesion.
复制标题
软骨细胞粘附的定量微孔测定。
DOI：
10.1016/0003-2697(91)90552-5
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发表时间：
1991
影响因子：
2.9
通讯作者：
McDevitt,CA
中科院分区：
文献类型：
--
作者：
Miller,RR;McDevitt,CA
通过测量由染料溴化3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四唑（MTT）产生的蓝色甲瓒产物的吸光度，建立了用于测量附着于微孔板中的基质的活关节软骨细胞的量的测定。当MTT浓度为1 mg/ml（100 μl/微孔），孵育时间为3 h时，蓝色甲瓒的产量最高。染料的吸光度与每个微孔中加入的细胞量呈线性相关。附着于粘附蛋白的活软骨细胞的数量可以使用该技术进行定量。
An assay for measuring the quantity of live articular chondrocytes attached to a substratum in microwell plates was established by measuring the absorbance of the blue formazan product generated from the dye 3-(4,5-dimethylthiazol-2-yl)-2,5-dyphenyl tetrazolium bromide (MTT). Blue formazan production was optimal at an MTT concentration of 1 mg/ml (100 μl per microwell) and an incubation period of 3 h. The absorbance of the dye was linearly related to the quantity of cells added per microwell. The number of live chondrocytes attached to adhesive proteins can be quantitated using this technique.