Use of hyaluronan-derived hydrogels for three-dimensional cell culture and tumor xenografts.
Use of hyaluronan-derived hydrogels for three-dimensional cell culture and tumor xenografts.
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DOI：
10.1002/0471143030.cb1014s40
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发表时间：
2008-09
影响因子：
--
通讯作者：
Prestwich, Glenn D
中科院分区：
文献类型：
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作者：
Serban, Monica A;Scott, Anna;Prestwich, Glenn D
体外三维（3-D）细胞培养的实践已经落后于传统的塑料表面二维（2-D）培养不能反映正常细胞生物学的认识。在生物学上，构成细胞外基质（ECM）的蛋白质和蛋白聚糖的复杂网络包围着每个细胞。为了概括正常的细胞行为，需要重建基本生物线索的支架（ECM类似物）。本单元描述了基于透明质酸和明胶交联衍生物的新型半合成ECM (sECM) Extracel的三维细胞培养和肿瘤工程应用。提供了一种简化的细胞包封和伪三维培养（在水凝胶之上）方案。此外，还介绍了使用这种sECM作为获得肿瘤异种移植物的载体，其采收率和肿瘤生长都有所提高。这些工程肿瘤可用于评估生理相关条件下的抗癌治疗。
The practice of in vitro three-dimensional (3-D) cell culture has lagged behind the realization that classical two-dimensional (2-D) culture on plastic surfaces fails to mirror normal cell biology. Biologically, a complex network of proteins and proteoglycans that constitute the extracellular matrix (ECM) surrounds every cell. To recapitulate the normal cellular behavior, scaffolds (ECM analogs) that reconstitute the essential biological cues are required. This unit describes the 3-D cell culture and tumor engineering applications of Extracel, a novel semisynthetic ECM (sECM), based on cross-linked derivatives of hyaluronan and gelatin. A simplified cell encapsulation and pseudo-3-D culturing (on top of hydrogels) protocol is provided. In addition, the use of this sECM as a vehicle to obtain tumor xenografts with improved take rates and tumor growth is presented. These engineered tumors can be used to evaluate anticancer therapies under physiologically relevant conditions.