EPR spectroscopic investigation ofpsoriatic finger nails
EPR spectroscopic investigation ofpsoriatic finger nails
复制标题
银屑病指甲的 EPR 光谱研究
DOI：
10.1111/srt.12068
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发表时间：
2013
期刊：
影响因子：
2.2
通讯作者：
Sawamura D
中科院分区：
文献类型：
--
作者：
Nakagawa K;Minakawa S;Sawamura D
研究背景甲襞病变是银屑病的常见特征，约半数患者有甲襞病变。然而，没有可行的光谱方法评估甲银屑病的变化和严重程度。EPR（电子顺磁共振）可能是可行的评估指甲的条件在psorias. Methods 5例指甲银屑病，（3女，2男）进行了检查。对指甲样品进行EPR测定。将小片指甲（1.5 × 5 mm 2）在约50 μM 5-DSA（5-doxylstearic acid）水溶液中于37 ℃下孵育约60 min。冲洗后，擦去多余的5-DSA溶液，指甲样品进行了测量EPR.ResultsEPR谱进行了分析，使用的强度比（快/慢）的两个运动在较低的磁场的峰值。我们观察到两个可区分的网站的基础上的EPR结果。此外，现代EPR计算进行了分析所获得的光谱。结论EPR结果表明，指甲中存在两个可区分的部位，即甲内银屑病区和甲外银屑病区。在指甲银屑病的情况下，脆性成分是对照的2~3倍。因此，EPR法被认为是一种新的和可靠的方法来评估指甲银屑病。
BackgroundNail lesions are common features of psoriasis and found in almost half of the patients. However, there is no feasible spectroscopic method evaluating changes and severity of nail psoriasis. EPR (electron paramagnetic resonance) might be feasible for evaluating nail conditions in the patients of psoriasis.MethodsFinger nails of five cases with nail psoriasis, (three females and two males) were examined. Nail samples were subjected to the EPR assay. The small piece of the finger nail (1.5 × 5 mm2) was incubated in ~50 μM 5‐DSA (5‐doxylstearic acid) aqueous solutions for about 60 min at 37°C. After rinsing and wiping off the excess 5‐DSA solution, the nail samples were measured by EPR.ResultsEPR spectra were analyzed using the intensity ratio (Fast/Slow) of the two motions at the peaks of the lower magnetic field. We observed two distinguishable sites on the basis of the EPR results. In addition, the modern EPR calculation was performed to analyze the spectra obtained. The nail psoriasis‐related region is 2~3 times higher than that of the control.ConclusionThe present EPR results show that there are two distinguishable sites in the nail. In the case of nail psoriasis, the fragile components are 2~3 times more than those of the control. Thus, the EPR method is thought to be a novel and reliable method of evaluating the nail psoriasis.