Metabolomic analysis of uremic toxins by liquid chromatography/electrospray ionization-tandem mass spectrometry
Metabolomic analysis of uremic toxins by liquid chromatography/electrospray ionization-tandem mass spectrometry
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DOI：
10.1016/j.jchromb.2009.11.040
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发表时间：
2010-06-15
影响因子：
3
通讯作者：
Niwa, Toshimitsu
中科院分区：
文献类型：
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作者：
Kikuchi, Kaori;Itoh, Yoshiharu;Niwa, Toshimitsu
我们应用液相色谱/电喷雾串联质谱技术对综合性小分子代谢物进行代谢组学分析（LC/ESI-MS/MS）和主成分分析鉴定慢性肾功能衰竭（CRF）大鼠血清中积累的尿毒症毒素通过5/6肾切除术产生CRF大鼠，硫酸吲哚酚被证明是区分CRF与正常的第一个主要血清代谢物，然后是硫酸苯酯、马尿酸和硫酸对甲苯酯。然后，我们通过LC/ESI-MS/MS的选择反应监测（SRM）测量硫酸吲哚酚、硫酸苯酯、马尿酸和对甲苯基硫酸酯的血清水平，并且证明与正常大鼠相比，这些血清水平在CRF大鼠中显著升高。随着肌酐清除率降低，代谢物的血清水平升高（C）2009 Elsevier B V。
We applied the metabolomic analysis of comprehensive small-molecular metabolites using liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS)and principal component analysis to identify uremic toxins accumulated in the serum of chronic renal failure (CRF) rats CRF rats were produced by 5/6-nephrectomy Indoxyl sulfate was demonstrated to be the first principal serum metabolite which differentiates CRF from not mal, followed by phenyl sulfate, hippuric acid and p-cresyl sulfate. Then, we measured the serum levels of indoxyl sulfate, phenyl sulfate, hippuric acid and p-cresyl sulfate by the selected reaction monitoring (SRM) of LC/ESI-MS/MS, and demonstrated that these serum levels were markedly increased in CRF rats as compared with normal rats As creatinine clearance decreased, the serum levels of the metabolites increased (C) 2009 Elsevier B V. All rights reserved