Genetic variation and genotype x environment interactions of phytosterol content in three doubled haploid populations of winter rapeseed
Genetic variation and genotype x environment interactions of phytosterol content in three doubled haploid populations of winter rapeseed
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DOI：
10.2135/cropsci2007.10.0578
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发表时间：
2008-05-01
期刊：
影响因子：
2.3
通讯作者：
Moellers, Christian
中科院分区：
文献类型：
--
作者：
Amar, Samija;Becker, Heiko C.;Moellers, Christian
植物甾醇是植物油的天然成分，以其降低胆固醇的特性而闻名。油菜籽（Brassica napus L.）是植物甾醇最丰富的天然来源之一。从基因上提高植物甾醇含量可以为菜籽油及其衍生产品提供附加值。本研究的目的是建立一种测定冬油菜种子中植物甾醇含量的气相色谱分析方法，以确定冬油菜3个双单倍体群体的遗传变异和基因型×环境互作，并估计植物甾醇与其它重要种子品质性状的相关性。这些种群在三到四个环境中进行了几年的测试。谷甾醇和菜油甾醇被检测为两种主要的植物甾醇，其次是油菜甾醇、燕麦甾醇和豆甾醇。发现大的差异，总植物甾醇含量（2.57至4.15克公斤（-1）种子），主要的遗传方差分量导致高遗传力范围从0.84至0.91。植物甾醇含量与油分含量无显著负相关，与蛋白质和硫甙含量无显著相关。大的遗传变异沿着而来的高遗传力表明，在不对油、蛋白质或硫苷含量产生负面影响的情况下，有效育种以提高植物甾醇含量和改变组成是可能的。
Phytosterols are natural constituents of vegetable oils and are known for their cholesterol-lowering properties. The oil of rapeseed (Brassica napus L.) is one of the richest natural sources of phytosterols. Genetically enhancing the phytosterol content could give an added value to the rapeseed oil and derived products. Our objectives were to develop a gas-liquid chromatographic method for the analysis of phytosterol content in seeds of oilseed rape, to determine the genetic variation and the genotype x environment interactions, and to estimate correlations between phytosterols and other important seed quality traits in three doubled haploid populations of winter rapeseed. The populations were tested during several years in three to four environments. Sitosterol and campesterol were detected as the two major phytosterols followed by brassicasterol, avenasterol, and stigmasterol. Large differences were found in total phytosterol content (2.57 to 4.15 g kg(-1) seed), with predominant genetic variance components resulting in high heritabilities ranging from 0.84 to 0.91. Phytosterol content was not negatively correlated with oil content and there were no close correlations to protein and glucosinolate content. The large genetic variation along with high heritabilities indicate that an effective breeding for enhanced phytosterol content and modified composition should be possible without negative impacts on oil, protein, or glucosinolate content.