Characterization of the yeast transcriptome
Characterization of the yeast transcriptome
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DOI：
10.1016/s0092-8674(00)81845-0
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发表时间：
1997-01-24
期刊：
影响因子：
64.5
通讯作者：
Kinzler, KW
中科院分区：
文献类型：
--
作者：
Velculescu, VE;Zhang, L;Kinzler, KW
我们已经使用基因表达的系列分析分析了从酵母基因组表达的基因组，本文称为转录组。对60，633个转录本的分析揭示了4，665个基因，每个细胞的表达水平从0.3到超过200个转录本不等。在这些基因中，有1981个已知功能，而2684个以前没有特征。位置信息与基因表达数据的整合允许产生染色体表达图谱，其鉴定转录活性的物理区域，并鉴定未单独通过序列信息预测的基因。这些研究提供了深入了解酵母中基因表达的全球模式，并证明了在真核生物中进行全基因组表达研究的可行性。
We have analyzed the set of genes expressed from the yeast genome, herein called the transcriptome, using serial analysis of gene expression. Analysis of 60,633 transcripts revealed 4,665 genes, with expression levels ranging from 0.3 to over 200 transcripts per cell. Of these genes, 1981 had known functions, while 2684 were previously uncharacterized. The integration of positional information with gene expression data allowed for the generation of chromosomal expression maps identifying physical regions of transcriptional activity and identified genes that had not been predicted by sequence information alone. These studies provide insight into global patterns of gene expression in yeast and demonstrate the feasibility of genome-wide expression studies in eukaryotes.