Nitrogen and Phosphorus Co-Doped Carbon Nanodots as a Novel Fluorescent Probe for Highly Sensitive Detection of Fe3+ in Human Serum and Living Cells
Nitrogen and Phosphorus Co-Doped Carbon Nanodots as a Novel Fluorescent Probe for Highly Sensitive Detection of Fe3+ in Human Serum and Living Cells
复制标题
氮磷共掺杂碳纳米点作为新型荧光探针，用于高灵敏检测人血清和活细胞中的 Fe3
DOI：
10.1021/acsami.6b01325
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发表时间：
2016-05-04
影响因子：
9.5
通讯作者：
Zhao, Shulin
中科院分区：
文献类型：
--
作者：
Shi, Bingfang;Su, Yubin;Zhao, Shulin
化学掺杂杂原子可以有效地调节碳量子点的物理化学和光化学性质。然而，多杂原子共掺杂碳纳米点的发展仍处于早期阶段。本论文采用水热合成法，以葡萄糖为碳源，氨水、磷酸为掺杂剂，合成了氮磷共掺杂的碳纳米点（N，P-CD）。与环糊精相比，多杂原子掺杂的环糊精在电子特性和表面化学活性方面都有显著的改善。因此，如上所述制备的N，P-CD表现出强的蓝色发射和对Fe 3+的灵敏响应。然后将基于N，P-CD的荧光传感器用于灵敏地测定Fe 3+，检测限为1.8 nM。值得注意的是，所制备的N，P-CD具有可忽略的细胞毒性，优异的生物相容性，和高的光稳定性。该方法还可用于复杂生物样品中Fe 3+的免标记检测和细胞内Fe 3+的荧光成像，在临床诊断和其他生物学相关研究中具有潜在的应用价值。
Chemical doping with heteroatoms can effectively modulate physicochemical and photochemical properties of carbon dots (CDs). However, the development of multi heteroatoms codoped carbon nanodots is still in its early stage. In this work, a facile hydrothermal Synthesis strategy was applied to synthesize multi heteroatoms (nitrogen and phosphorus) codoped carbon nanodots (N,P-CDs) using glucose as carbon source, and ammonia, phosphoric acid as dopant, respectively. Compared with CDs, the multi heteroatoms doped CDs resulted in dramatic improvement in the electronic characteristics and surface chemical activities. Therefore, the N,P-CDs prepared as described above exhibited a strong blue emission and a sensitive response to Fe3+. The N,P-CDs based fluorescent sensor was then applied to sensitively determine Fe3+ with a detection limit of 1.8 nM. Notably, the prepared N,P-CDs possessed negligible cytotoxicity, excellent biocompatibility, and high photostability. It was also applied for label-free detection of Fe3+ in complex biological samples and the fluorescence imaging of intracellular Fe3+, which indicated its potential applications in clinical diagnosis and other biologically related study.