γδ T lymphocytes are recruited into the inflamed uterus of bitches suffering from pyometra
γδ T lymphocytes are recruited into the inflamed uterus of bitches suffering from pyometra
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DOI：
10.1016/j.tvjl.2012.05.024
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发表时间：
2012-12-01
影响因子：
2.2
通讯作者：
Faldyna, M.
中科院分区：
文献类型：
--
作者：
Bartoskova, A.;Turanek-Knotigova, P.;Faldyna, M.
关于免疫系统细胞在犬子宫中的存在，人们知之甚少。这项研究的目的是产生关于健康犬子宫中存在的淋巴细胞亚群的信息，这些淋巴细胞亚群是在子宫积脓引起的炎症条件下招募的。免疫组织化学和流式细胞仪检测发现，子宫积液标本中有大量的γ-Delta T淋巴细胞，其主要原因是γ-Delta(+)/CD8(-)T淋巴细胞的募集。对来自健康子宫和子宫积脓的子宫样本中编码特定细胞因子/趋化因子的基因的相对表达进行了评估。促炎症细胞因子(包括IL-1β、TNF-α、IL-8、IL-17和干扰素-γ)和趋化因子(包括CXCL10、CCL4和CCL5)在子宫积液标本中表达上调，证实存在炎症。相比之下，体内平衡趋化因子CCL25和抗炎细胞因子IL-10的表达分别下调和不变。(C)2012爱思唯尔有限公司。保留所有权利。
Very little is known about the occurrence of immune system cells in the canine uterus. The aim of this study was to generate information about lymphocyte subsets that are present in the healthy canine uterus and that are recruited under inflammatory conditions caused by pyometra. Using immunohistochemistry and flow cytometry, a significant influx of gamma delta T lymphocytes was found in pyometra samples mainly due to recruitment of gamma delta(+)/CD8(-) T lymphocytes. The relative expression of genes encoding selected cytokines/chemokines was evaluated in samples from healthy and pyometra-affected uteri. Expression of pro-inflammatory cytokines (including IL-1 beta, TNF-alpha, IL-8, IL-17 and IFN-gamma) and chemokines (including CXCL10, CCL4 and CCL5) was upregulated in pyometra samples confirming the presence of inflammation. In contrast, the expression of the homeostatic chemokine CCL25 and of the anti-inflammatory cytokine IL-10 was downregulated and unchanged, respectively. (C) 2012 Elsevier Ltd. All rights reserved.